Construct: ORF ccsbBroad304_03052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003277.1_s304c1, BRDN0000409726
- Derived from:
- ccsbBroadEn_03052
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL42 (28977)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03052
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28977 | MRPL42 | mitochondrial ribosomal pro... | NM_014050.4 | 100% | 100% | |
| 2 | human | 28977 | MRPL42 | mitochondrial ribosomal pro... | NM_172177.5 | 100% | 100% | |
| 3 | human | 359821 | MRPL42P5 | mitochondrial ribosomal pro... | NR_002208.1 | 53.4% | (many diffs) | |
| 4 | human | 28977 | MRPL42 | mitochondrial ribosomal pro... | NR_038160.2 | 2.7% | 1_135del;206_225del;582_15577del | |
| 5 | human | 28977 | MRPL42 | mitochondrial ribosomal pro... | NR_038159.2 | 2.7% | 1_138del;209_228del;585_15580del | |
| 6 | human | 28977 | MRPL42 | mitochondrial ribosomal pro... | NR_038161.2 | 2.7% | 1_135del;206_310del;667_15662del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtagctgca gtaaaatggg tgatgtcaaa gagaactatc ttgaaacatt 121 tatttccagt ccaaaatgga gctttatatt gtgtttgtca taaatctacg tattctcctc 181 taccagatga ctataattgc aacgtagagc ttgctctgac ttctgatggc aggacaatag 241 tatgctacca cccttctgtg gacattccat atgaacacac aaaacctatc cctcggccag 301 atcctgtgca taataatgaa gaaacacatg atcaagtgct gaaaaccaga ttggaagaaa 361 aagttgaaca ccttgaggaa ggacctatga tagaacaact tagcaaaatg ttctttacta 421 ctaagcaccg ttggtatcct catggacggt atcacagatg tcgtaagaat ctgaatcctc 481 caaaagacag atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 541 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 601 caatcaacct ctggatta