Construct: ORF ccsbBroad304_03102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002700.1_s304c1, BRDN0000461195
- Derived from:
- ccsbBroadEn_03102
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBIAD1 (29914)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03102
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29914 | UBIAD1 | UbiA prenyltransferase doma... | NM_013319.2 | 100% | 100% | |
| 2 | human | 29914 | UBIAD1 | UbiA prenyltransferase doma... | NM_001330349.1 | 62.1% | 61.2% | (many diffs) |
| 3 | human | 29914 | UBIAD1 | UbiA prenyltransferase doma... | NM_001330350.1 | 52.9% | 52% | 529_530ins39;537_538ins438 |
| 4 | human | 29914 | UBIAD1 | UbiA prenyltransferase doma... | XR_946616.3 | 43.5% | (many diffs) | |
| 5 | mouse | 71707 | Ubiad1 | UbiA prenyltransferase doma... | NM_027873.2 | 88.8% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1081
- ORF length:
- 1014
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggccatgg cggcctctca ggtcctgggg gagaagatta acatcctgtc gggagagact 121 gtcaaagctg gggacaggga cccgctgggg aacgactgtc ccgagcaaga taggctcccc 181 cagcgctcct ggaggcagaa gtgtgcctcc tacgtgttgg ccctgaggcc ctggagcttc 241 agtgcctcac tcacaccggt ggccctgggc agtgcccttg cctacagatc ccacggtgtc 301 ctggatccca ggctcttggt gggttgtgcc gtggctgtcc tggctgtgca cggggccggt 361 aatttggtca acacttacta tgacttttcc aagggcattg accacaaaaa gagtgatgac 421 aggacacttg tggaccgaat cttggagccg caggatgtcg tccggttcgg agtcttcctc 481 tacacgttgg gctgcgtctg tgccgcttgc ctctactacc tgtcccctct gaaactggag 541 cacttggctc ttatctactt tggaggcctg tctggctcct ttctctacac aggaggaatt 601 ggattcaagt acgtggctct gggagacctc atcatcctca tcacttttgg cccgctggct 661 gtgatgttcg cctacgccat ccaggtgggg tccctggcca tcttcccact ggtctatgcc 721 atccccctcg ccctcagcac cgaggccatt ctccattcca acaacaccag ggacatggag 781 tccgaccggg aggctggtat cgtcacgctg gccatcctca tcggccccac gttctcctac 841 attctctaca acacactgct cttcctgccc tacctggtct tcagcatcct ggccacacac 901 tgcaccatca gcctggcact ccccctgctt accattccca tggccttctc ccttgagaga 961 cagtttcgaa gccaggcctt caacaaactg ccccagagga ctgccaagct caacctcctg 1021 ctgggacttt tctatgtctt tggcatcatt ctggcaccag caggcagtct gcccaaaatt 1081 ttgccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc 1141 gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc 1201 tggatta