Construct: ORF ccsbBroad304_03112

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003267.1_s304c1, BRDN0000460411
Derived from:
ccsbBroadEn_03112
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NENF (29937)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03112
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29937 NENF neudesin neurotrophic factor NM_013349.5 100% 100%
2 human 29937 NENF neudesin neurotrophic factor NR_026598.2 49.6% 1_24del;200_201ins61;480_855del
3 mouse 66208 Nenf neuron derived neurotrophic... NM_025424.2 88.1% 90.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
582
ORF length:
516
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gggccccgcg ccgcggcggc ggctgcggcc gctggcagcg ctggccctgg
121tcctggcgct ggccccgggg ctgcccacag cccgggccgg gcagacaccg cgccctgccg
181agcgggggcc cccagtgcgg cttttcaccg aggaggagct ggcccgctat ggcggggagg
241aggaagatca gcccatctac ttggcagtga agggagtggt gtttgatgtc acctccggaa
301aggagtttta tggacgagga gccccctaca atgccttgac ggggaaggac tccactagag
361gggtagccaa gatgtccttg gatcctgcag acctcaccca tgacactacg ggtctcacgg
421ccaaggaact ggaggccctg gatgaggtct tcaccaaagt gtacaaagcc aaatacccca
481tcgtcggcta cactgcccgg agaattctca atgaggatgg cagccctaac ctggacttca
541agcctgaaga ccagccccat tttgacatca aggatgagtt ctgcccaact ttcttgtaca
601aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
661ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)