Construct: ORF ccsbBroad304_03114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008926.1_s304c1, BRDN0000401647
- Derived from:
- ccsbBroadEn_03114
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PURG (29942)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03114
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29942 | PURG | purine rich element binding... | NM_001323311.1 | 100% | 100% | |
| 2 | human | 29942 | PURG | purine rich element binding... | NM_013357.2 | 100% | 100% | |
| 3 | human | 29942 | PURG | purine rich element binding... | NM_001015508.3 | 87.3% | 85.1% | (many diffs) |
| 4 | human | 29942 | PURG | purine rich element binding... | NM_001323312.2 | 87.3% | 85.1% | (many diffs) |
| 5 | mouse | 75029 | Purg | purine-rich element binding... | NM_152821.2 | 93.2% | 96.2% | (many diffs) |
| 6 | mouse | 75029 | Purg | purine-rich element binding... | XM_006509206.2 | 93.2% | 96.2% | (many diffs) |
| 7 | mouse | 75029 | Purg | purine-rich element binding... | XM_017312994.2 | 83% | 82.7% | (many diffs) |
| 8 | mouse | 75029 | Purg | purine-rich element binding... | NM_001098233.1 | 82.1% | 81.8% | (many diffs) |
| 9 | mouse | 75029 | Purg | purine-rich element binding... | XM_006509209.4 | 82.1% | 81.8% | (many diffs) |
| 10 | mouse | 75029 | Purg | purine-rich element binding... | XM_011242165.3 | 82.1% | 80.8% | (many diffs) |
| 11 | mouse | 75029 | Purg | purine-rich element binding... | XM_011242166.3 | 82.1% | 80.8% | (many diffs) |
| 12 | mouse | 75029 | Purg | purine-rich element binding... | XM_006509205.2 | 79.7% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1110
- ORF length:
- 1041
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaagagcc aggcgaaggg gaggcggcgg cggccgcggc cgcggaggca 121 agaatgtagg gggctctggc ctaagcaaga gtagactcta tccccaggcc cagcactccc 181 actaccccca ctacgcggcc tcagccaccc ctaatcaggc cgggggcgca gccgaaatcc 241 aggagctggc ctccaaacga gtggacatcc agaaaaagag gttttaccta gacgtgaagc 301 aaagctcccg gggccgcttc ctaaagatag ccgaagtctg gatagggaga ggccggcagg 361 acaacatcag aaagagtaaa ctgaccctct ccctgtctgt ggcagcggag ctgaaggact 421 gtctagggga cttcatcgag cactatgccc acctgggcct gaaaggccac cggcaagagc 481 atggccacag caaagagcaa ggctccagaa ggaggcagaa gcactcggca ccctccccac 541 cagtctcggt ggggtccgaa gagcatcctc acagtgtcct gaaaacagac tatatcgaga 601 gggacaatag gaaatattac ctagacctaa aggaaaatca gcggggtcgc ttcctacgga 661 ttagacaaac catgatgcgg gggactggca tgataggtta ttttggccac agtttgggcc 721 aagaacagac tattgtcctc ccagcacaag gaatgattga gtttcgtgat gccttggttc 781 agctgattga agactatggc gaaggagaca tagaagaacg aagaggtgga gacgatgacc 841 cgcttgaact cccagagggg acttctttca gagtggacaa taaaaggttc tactttgatg 901 tgggctctaa taaatatgga attttcctga aggtaagtga ggtgagacca ccttaccgta 961 atactattac tgttccattc aaagcttgga caaggtttgg ggagaatttt atcaagtatg 1021 aagaagagat gaggaaaatt tgcaacagcc ataaagaaaa gagaatggat ggcagaaagg 1081 ccagtggtga agaacaagaa tgcctcgact tgccaacttt cttgtacaaa gtggttggta 1141 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1201 ggtggcgcgt taagtcgaca atcaacctct ggatta