Construct: ORF ccsbBroad304_03157
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012095.1_s304c1, BRDN0000400397
- Derived from:
- ccsbBroadEn_03157
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAT1 (50852)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03157
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50852 | TRAT1 | T cell receptor associated ... | NM_016388.4 | 100% | 100% | |
| 2 | human | 50852 | TRAT1 | T cell receptor associated ... | NM_001317747.2 | 79.9% | 79% | 0_1ins97;5_5delCinsGGAAAAGCAACGACA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 624
- ORF length:
- 558
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggaatctct gggtgcccct ttttcctctg gggacttcta gcattgttgg 121 gcttggcttt ggttatatca ctgatcttca atatttccca ctatgtggaa aagcaacgac 181 aagataaaat gtacagctac tccagtgacc acaccagggt tgatgagtat tatattgaag 241 acacaccaat ttatggtaac ttagatgata tgatttcaga accaatggat gaaaattgct 301 atgaacaaat gaaagcccga ccagagaaat ctgtaaataa gatgcaggaa gccaccccat 361 ctgcacaggc aaccaatgaa acacagatgt gctacgcctc acttgatcac agcgttaagg 421 ggaagcgtag aaagcccagg aaacagaata ctcatttctc agacaaggat ggagatgagc 481 aactacatgc aatagatgcc agcgtttcta agaccacctt agtagacagt ttctccccag 541 aaagccaggc agtagaggaa aacattcatg atgatcccat cagactgttt ggattgatcc 601 gtgctaagag agaacctata aactacccaa ctttcttgta caaagtggtt ggtaagccta 661 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 721 gcgttaagtc gacaatcaac ctctggatta