Construct: ORF ccsbBroad304_03252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010587.1_s304c1, BRDN0000405738
- Derived from:
- ccsbBroadEn_03252
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX16 (51241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03252
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51241 | COX16 | cytochrome c oxidase assemb... | NM_016468.7 | 100% | 100% | |
| 2 | human | 51241 | COX16 | cytochrome c oxidase assemb... | NM_001204090.2 | 77.3% | 77.3% | 69_70ins72 |
| 3 | human | 100529257 | SYNJ2BP-COX16 | SYNJ2BP-COX16 readthrough | NM_001202548.2 | 54.4% | 45% | (many diffs) |
| 4 | mouse | 66272 | Cox16 | cytochrome c oxidase assemb... | NM_025461.6 | 85.5% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgcacccgcg gtgatgcgtg cttttcgcaa gaacaagact ctcggctatg 121 gagtccccat gttgttgctg attgttggag gttcttttgg tcttcgtgag ttttctcaaa 181 tccgatatga tgctgtgaag agtaaaatgg atcctgagct tgaaaaaaaa ctgaaagaga 241 ataaaatatc tttagagtcg gaatatgaga aaatcaaaga ctccaagttt gatgactgga 301 agaatattcg aggacccagg ccttgggaag atcctgacct cctccaagga agaaatccag 361 aaagccttaa gactaagaca acttgcccaa ctttcttgta caaagtggtt ggtaagccta 421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 481 gcgttaagtc gacaatcaac ctctggatta