Construct: ORF ccsbBroad304_03415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006464.1_s304c1, BRDN0000408555
- Derived from:
- ccsbBroadEn_03415
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFOD1 (54438)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03415
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | NM_018988.4 | 100% | 100% | |
| 2 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | NM_001242628.1 | 73.5% | 73.5% | 0_1ins309 |
| 3 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | NM_001242630.1 | 73.5% | 73.5% | 0_1ins309 |
| 4 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | XM_011514699.2 | 73.5% | 73.5% | 0_1ins309 |
| 5 | human | 54438 | GFOD1 | glucose-fructose oxidoreduc... | NM_001242629.1 | 28.5% | 23.8% | (many diffs) |
| 6 | mouse | 328232 | Gfod1 | glucose-fructose oxidoreduc... | NM_001033399.4 | 90.7% | 98.2% | (many diffs) |
| 7 | mouse | 328232 | Gfod1 | glucose-fructose oxidoreduc... | XM_006516932.3 | 66.5% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1239
- ORF length:
- 1170
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcttcccggg gtgggcgtgt tcggcaccag cctcacggcc cgtgtcatca 121 tcccgctgct gaaagacgag ggcttcgcgg tgaaggcgct gtggggccgc acgcaggaag 181 aagcggagga gctggccaag gagatgagtg tccccttcta cactagccgc attgatgagg 241 tgctgctgca tcaggacgtg gacttggtgt gcattaacct gccgccgccc ctcaccagac 301 agatcgctgt caaaacccta ggcatcggca agaacgtcat ctgcgaccgc acggccacgc 361 cgctggacgc tttccgcatg acctcggccg cccactacta ccccaagctc atgagcatca 421 tgggcaacgt gctgcgcttc ctgccggctt tcgtgcgcat gaagcagctg atcgaggagg 481 gctacgtggg cgagccgctg gtgtgtgagg tgcaggtgca cggcggcagc ctgctgggca 541 agaagtacaa ctggagctgc gacgacttga tgggcggcgg cggcctgcac tccgtgggca 601 cctacatcat cgacctgctc accttcctca ccggccaaaa ggccgtcaag gtccacgggc 661 tgctcaagac cttcgtgaag cagactgacc acatcaaggg catccgacag atcaccagcg 721 atgacttctg caccttccag atggtgctgg agggcggggt gtgctgcacc gtcaccctca 781 acttcaacgt gcccggcgag ttcaagcagg atgtcactgt ggtgggctca gccgggcgcc 841 tgctggccgt gggcaccgac ctgtacgggc agcgcaacag cgccccggag caggagctgc 901 tggtgcagga cgccacgccg gtgagcaact ccctgcttcc ggagaaggcc ttcagcgaca 961 tcccctcgcc ctacctgcgc ggcaccatca agatgatgca ggcggtgcgc caggccttcc 1021 aggaccagga cgaccggcgc acgtgggatg ggcggcccct caccatggcc gccaccttcg 1081 acgactgcct gtatgccttg tgcgtggtgg acaccatcaa gaggtccagc cagacgggcg 1141 agtggcagaa cattgccatc atgaccgagg agccggagct gagccccgcc tacctgatca 1201 gcgaggccat gcgccgcagc aggatgtccc tctactgttt gccaactttc ttgtacaaag 1261 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1321 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta