Transcript: Mouse NM_001033399.4

Mus musculus glucose-fructose oxidoreductase domain containing 1 (Gfod1), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Gfod1 (328232)
Length:
6654
CDS:
676..1848

Additional Resources:

NCBI RefSeq record:
NM_001033399.4
NBCI Gene record:
Gfod1 (328232)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001033399.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235591 TCATCATCCCGCTGCTGAAAG pLKO_005 722 CDS 100% 10.800 15.120 N GFOD1 n/a
2 TRCN0000265573 GTAGTCTGCTGGGCAAGAAAT pLKO_005 1133 CDS 100% 13.200 10.560 N Gfod1 n/a
3 TRCN0000254593 CCGTTACCCTGAACTTCAATG pLKO_005 1376 CDS 100% 10.800 8.640 N Gfod1 n/a
4 TRCN0000254594 TGTCATTGTCTAGTCTATATT pLKO_005 6392 3UTR 100% 15.000 10.500 N Gfod1 n/a
5 TRCN0000254592 ACCTCTATGGACAGCGCAATA pLKO_005 1466 CDS 100% 10.800 7.560 N Gfod1 n/a
6 TRCN0000254595 TACGCATGAAGCAGCTGATTG pLKO_005 1061 CDS 100% 10.800 7.560 N Gfod1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001033399.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03415 pDONR223 100% 90.7% 98.2% None (many diffs) n/a
2 ccsbBroad304_03415 pLX_304 0% 90.7% 98.2% V5 (many diffs) n/a
3 TRCN0000467567 TAGCGCTTCTCAGTGGAGTGGGCT pLX_317 35.3% 90.7% 98.2% V5 (many diffs) n/a
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