Construct: ORF ccsbBroad304_03435
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007706.1_s304c1, BRDN0000406859
- Derived from:
- ccsbBroadEn_03435
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNL3L (54552)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03435
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54552 | GNL3L | G protein nucleolar 3 like | NM_001184819.2 | 100% | 100% | |
| 2 | human | 54552 | GNL3L | G protein nucleolar 3 like | NM_019067.6 | 100% | 100% | |
| 3 | mouse | 237107 | Gnl3l | guanine nucleotide binding ... | NM_001168600.1 | 85.5% | 86.8% | (many diffs) |
| 4 | mouse | 237107 | Gnl3l | guanine nucleotide binding ... | NM_198110.2 | 85.5% | 86.8% | (many diffs) |
| 5 | mouse | 237107 | Gnl3l | guanine nucleotide binding ... | XM_006528805.3 | 85.5% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1812
- ORF length:
- 1746
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gaaacttaga cacaaaaata aaaagccagg tgaaggttcc aagggccaca 121 agaagataag ttggccctac cctcagcctg caaagcaaaa tgggaagaaa gcaacctcca 181 aagtgccctc tgcacctcat tttgttcacc ccaatgatca tgccaatcga gaggctgaat 241 taaagaagaa gtgggttgag gagatgaggg agaagcagca agccgcccgg gagcaagaaa 301 gacaaaaacg caggaccatt gagagctact gtcaggatgt cctaagacgc caggaggagt 361 ttgagcataa ggaggaagtt ttgcaggaat taaatatgtt tcctcagctg gatgacgagg 421 ccacgaggaa ggcttattac aaggagttcc gtaaggtggt ggaatactct gatgtgattc 481 tggaagtcct ggatgccaga gacccattag gctgccgctg cttccaaatg gaggaggctg 541 tcctgcgagc acaaggcaac aagaagctgg tcctggtctt gaacaagatt gacctggtcc 601 ccaaggaggt tgtggagaaa tggctggatt accttcggaa tgagttgcca accgtggctt 661 tcaaggccag tacccagcat caggtcaaaa acctgaatcg ttgcagtgtg ccagtagatc 721 aggcctctga gtcactgctg aaaagcaaag cctgctttgg agctgaaaac ctcatgaggg 781 ttctggggaa ctattgccgc cttggtgaag tgcgcaccca cattcgtgtg ggtgttgtgg 841 gtcttcccaa tgttgggaag agcagcctga tcaatagcct gaagcgcagc cgcgcatgca 901 gcgtgggagc tgttcctgga attaccaaat tcatgcagga ggtctacctg gacaagttca 961 tccggctctt ggatgctcca ggcattgtcc cagggcccaa ctcagaggtg ggcaccatcc 1021 tgcgtaactg cgtccacgtg cagaagctgg cagaccctgt gaccccagtg gagaccatcc 1081 tgcagcgctg caacctggag gagatttcca actattatgg cgtctctggg ttccagacca 1141 ctgagcactt tctgacggca gtggcccacc gtttggggaa gaagaagaag ggaggcttat 1201 atagtcagga acaggcggcc aaagctgtcc tagctgactg ggtgagcggg aagatcagct 1261 tctatatacc accaccagcc actcacactc tgcccaccca tctcagtgct gagatcgtta 1321 aggaaatgac cgaggtcttt gacatcgagg atactgagca ggccaatgaa gacaccatgg 1381 aatgcttggc caccggagaa tctgatgagc tgttgggtga cacggaccca cttgaaatgg 1441 agatcaagtt gctccattct ccgatgacga aaatagcaga tgccattgaa aataaaacca 1501 ccgtgtataa gattggagat ctcactgggt attgcaccaa tccgaaccgt catcagatgg 1561 ggtgggctaa acgcaatgtg gaccaccgcc ctaagagcaa cagtatggtg gatgtctgct 1621 cagtggaccg ccgctcagtg ctgcagagga tcatggagac ggaccccctg caacagggcc 1681 aggctctggc atctgccctg aaaaataaga agaagatgca gaaacgtgca gataaaatcg 1741 ccagcaagct gtctgattcc atgatgtctg ctctcgacct ctctggcaat gctgatgatg 1801 gtgttggtga ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1861 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1921 caatcaacct ctggatta