Construct: ORF ccsbBroad304_03458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005611.1_s304c1, BRDN0000409983
- Derived from:
- ccsbBroadEn_03458
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH4 (54784)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03458
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54784 | ALKBH4 | alkB homolog 4, lysine deme... | NM_017621.4 | 100% | 100% | |
| 2 | human | 54784 | ALKBH4 | alkB homolog 4, lysine deme... | XM_005250464.3 | 75.8% | 75.8% | 0_1ins219 |
| 3 | human | 54784 | ALKBH4 | alkB homolog 4, lysine deme... | XM_005250465.2 | 54.3% | 54.3% | 0_1ins414 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggctgcc gccgagaccc ccgaagtcct tcgggaatgc ggttgcaagg 121 gcatccggac ctgtctgatc tgcgagcggc agcgcggcag tgacccgccc tgggagctgc 181 ccccagcgaa aacataccgt ttcatttact gctccgacac cggctgggcc gtgggcacag 241 aggagtctga ctttgagggc tgggccttcc ccttcccagg agtgatgctg atcgaggact 301 ttgtgacccg ggaggaagaa gccgagttgg tgcggctcat ggaccgtgac ccctggaagc 361 tctcccagtc tggacggagg aagcaggact atggccccaa agtcaacttt cggaaacaga 421 agctaaagac cgagggcttc tgcggcctcc ccagcttcag ccgggaggtg gtgcggagga 481 tgggcctcta cccggggctg gagggcttcc ggcccgtcga gcagtgcaac ctggactact 541 gccccgagcg gggctctgcc attgaccccc acctggacga cgcctggctg tggggggagc 601 ggctggtcag cctcaacctc ctgtccccca ccgtgctgtc catgtgtcgg gaggcgcccg 661 ggagcctgct cctctgctcg gccccgtcgg ctgccccgga ggccttggtg gacagcgtga 721 tagcacccag ccggtcggtg ctatgccagg aggtggaggt ggccatcccc ttacccgccc 781 gctccctgct ggtcctcacc ggggcggcac ggcaccagtg gaagcatgcc atccaccgca 841 gacacatcga ggcccgccgc gtctgcgtca ctttccggga gctgtcggct gagtttggcc 901 ctggagggag gcagcaagag ctgggccagg aactgctgcg gatcgccctc tccttccagg 961 gaagacccgt gtgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1021 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1081 caatcaacct ctggatta