Construct: ORF ccsbBroad304_03482

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014016.1_s304c1, BRDN0000406232
Derived from:
ccsbBroadEn_03482
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LIME1 (54923)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03482
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54923 LIME1 Lck interacting transmembra... NM_017806.4 100% 100%
2 human 54923 LIME1 Lck interacting transmembra... NM_001305654.1 59.6% 30.4% 179_180ins35;528_529ins322
3 human 54923 LIME1 Lck interacting transmembra... NM_001305655.2 59.6% 30.4% 179_180ins35;528_529ins322
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
951
ORF length:
885
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg gctgccagtg tcctgggccc ctcctgccct ctgggttcta gggtgctgcg
121ccctgctcct ctcgctgtgg gcgctgtgca cagcctgccg caggcccgag gacgctgtag
181cccccaggaa gagggcgcgg aggcagcggg cgaggctgca gggcagtgcg acggcggcgg
241aagcgtccct actgaggcgg acccacctct gctccctcag caagtcggac accagactgc
301acgagctgca ccggggcccg cgcagcagca gggccctgcg gcctgccagc atggatctcc
361tgcgcccaca ctggctggag gtgtccaggg acatcaccgg accgcaggca gccccctctg
421ccttcccaca ccaggagctg ccccgggctc tgccggcagc tgcagccacc gcagggtgcg
481ctggcctcga ggccacctat tccaacgtgg ggctggcggc ccttcccggg gtcagcctgg
541cggccagccc tgtggtggcc gagtatgccc gcgtccagaa gcgcaaaggg acccatcgca
601gtccccaaga gccacagcag gggaagactg aggtgacccc ggccgctcag gtggacgtcc
661tgtactccag ggtctgcaag cctaaaagga gggacccagg acccaccaca gacccgctgg
721accccaaggg ccagggagcg attctggccc tggcgggtga cctggcctac cagaccctcc
781cgctcagggc cctggatgtg gacagcggcc ccctggaaaa cgtgtatgag agcatccggg
841agctggggga ccctgctggc aggagcagca cgtgcggggc tgggacgccc cctgcttcca
901gctgccccag cctagggagg ggctggagac ccctccctgc ctccctgccc tgcccaactt
961tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
1021agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)