Transcript: Human NM_001305655.2

Homo sapiens Lck interacting transmembrane adaptor 1 (LIME1), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-01
Taxon:
Homo sapiens (human)
Gene:
LIME1 (54923)
Length:
1122
CDS:
67..597

Additional Resources:

NCBI RefSeq record:
NM_001305655.2
NBCI Gene record:
LIME1 (54923)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001305655.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244760 TCCAGGGTCTGCAAGCCTAAA pLKO_005 632 3UTR 100% 10.800 7.560 N LIME1 n/a
2 TRCN0000244759 GAACACTCAAGGACCTGTGCT pLKO_005 918 3UTR 100% 2.640 1.848 N LIME1 n/a
3 TRCN0000244758 GTATGAGAGCATCCGGGAGCT pLKO_005 790 3UTR 100% 0.720 0.504 N LIME1 n/a
4 TRCN0000257009 CTCAGGTGGACGTCCTGTACT pLKO_005 612 3UTR 100% 1.650 0.990 N LIME1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001305655.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03482 pDONR223 100% 59.6% 30.4% None 179_180ins35;528_529ins322 n/a
2 ccsbBroad304_03482 pLX_304 0% 59.6% 30.4% V5 179_180ins35;528_529ins322 n/a
3 TRCN0000467426 TACATCCCGGTGGAATTAGATAGG pLX_317 37.5% 59.6% 30.4% V5 179_180ins35;528_529ins322 n/a
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