Construct: ORF ccsbBroad304_03498

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014206.1_s304c1, BRDN0000402901
Derived from:
ccsbBroadEn_03498
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GEMIN8 (54960)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03498
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54960 GEMIN8 gem nuclear organelle assoc... NM_001042479.2 100% 100%
2 human 54960 GEMIN8 gem nuclear organelle assoc... NM_001042480.2 100% 100%
3 human 54960 GEMIN8 gem nuclear organelle assoc... NM_017856.3 100% 100%
4 human 54960 GEMIN8 gem nuclear organelle assoc... XM_017029617.2 100% 100%
5 human 54960 GEMIN8 gem nuclear organelle assoc... XM_017029618.2 100% 100%
6 human 54960 GEMIN8 gem nuclear organelle assoc... XM_005274555.3 70.5% 70.5% 1_303del
7 human 54960 GEMIN8 gem nuclear organelle assoc... XR_001755705.2 43.1% 1_173del;900_1681del
8 human 54960 GEMIN8 gem nuclear organelle assoc... XR_001755704.2 42.5% 1_173del;900_1708del
9 human 54960 GEMIN8 gem nuclear organelle assoc... XR_001755702.1 40.5% 1_257del;984_1792del
10 human 492303 GEMIN8P4 gem nuclear organelle assoc... NR_002830.1 39.2% (many diffs)
11 human 54960 GEMIN8 gem nuclear organelle assoc... XR_001755703.1 35.3% 1_257del;984_2054del
12 human 54960 GEMIN8 gem nuclear organelle assoc... XR_950466.3 13.7% 1_3811del;4538_5299del
13 human 54960 GEMIN8 gem nuclear organelle assoc... XR_950464.3 13.5% 1_3811del;4538_5348del
14 human 54960 GEMIN8 gem nuclear organelle assoc... XR_950467.3 12.9% 1_3811del;4538_5608del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
792
ORF length:
726
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agcggtaaag gcatcaacat cgaaagctac caggccttgg tattctcatc
121cggtatatgc aagatactgg caacattatc atcaagcaat ggcttggatg caaagccatc
181acaatgccta caggaaggcc gtggaatcct gtttcaatct tccatggtac ttaccttctg
241cgcttcttcc ccaaagctct tacgataatg aggctgcgta tcctcagtcc ttctatgacc
301atcatgtggc ctggcaggac tacccctgca gttcttcaca tttcagaaga tctgggcagc
361atccacgtta cagcagtagg atccaggcat ccacaaaaga agaccaagct ttgtccaaag
421aggaagagat ggagactgag tcagatgcag aggtagaatg tgacctgagc aatatggaaa
481tcactgaaga gctccgccag tactttgcag agaccgagag gcatagagaa gaacgacggc
541ggcagcagca gctggatgca gagcgcctgg acagctatgt gaacgctgac cacgacctgt
601actgcaacac ccgccggtcg gtagaagccc caactgagag gcctggtgag cggcgccagg
661ccgagatgaa gcgtttgtac ggggacagtg ctgccaagat ccaagccatg gaggccgcgg
721tgcagctgag ctttgacaag cactgtgacc gaaagcagcc caagtactgg ccggtcatcc
781ccctgaagtt ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
841tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
901caatcaacct ctggatta

Download FASTA (ORF) (Full)