Construct: ORF ccsbBroad304_03628

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010250.1_s304c1, BRDN0000462185
Derived from:
ccsbBroadEn_03628
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCYL2 (55681)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03628
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55681 SCYL2 SCY1 like pseudokinase 2 NM_001317784.1 100% 100%
2 human 55681 SCYL2 SCY1 like pseudokinase 2 NM_017988.6 100% 100%
3 human 55681 SCYL2 SCY1 like pseudokinase 2 NM_001330253.1 99.5% 99.5% 1272_1283del
4 human 55681 SCYL2 SCY1 like pseudokinase 2 NM_001330254.1 99.5% 99.5% 1272_1283del
5 human 55681 SCYL2 SCY1 like pseudokinase 2 NM_001330256.1 81% 81% 0_1ins519;753_764del
6 mouse 213326 Scyl2 SCY1-like 2 (S. cerevisiae) NM_198021.2 88.7% 93% (many diffs)
7 mouse 213326 Scyl2 SCY1-like 2 (S. cerevisiae) NM_001310704.1 88.7% 92.9% (many diffs)
8 mouse 213326 Scyl2 SCY1-like 2 (S. cerevisiae) XM_011243394.2 88.4% 92.6% (many diffs)
9 mouse 213326 Scyl2 SCY1-like 2 (S. cerevisiae) XM_006513458.3 88.3% 92.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2853
ORF length:
2787
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gtccatgctt aataaattga agagtactgt tacaaaagta acagctgatg
121tcactagtgc tgtaatggga aatcctgtca ctagagaatt tgatgttggt cgacacattg
181ccagtggtgg caatgggcta gcttggaaga tttttaatgg cacaaaaaag tcaacaaagc
241aggaagtggc agtttttgtc tttgataaaa aactgattga caagtatcaa aaatttgaaa
301aggatcaaat cattgattct ctaaaacgag gagtccaaca gttaactcgg cttcgacacc
361ctcgacttct tactgtccag catcctttag aagaatccag ggattgcttg gcattttgta
421cagaaccagt ttttgccagt ttagccaatg ttcttggtaa ctgggaaaat ctaccttccc
481ctatatctcc agacattaag gattataaac tttatgatgt agaaaccaaa tatggtttgc
541ttcaggtttc tgaaggattg tcattcttgc atagcagtgt gaaaatggtg catggaaata
601tcactcctga aaatataatt ttgaataaaa gtggagcctg gaaaataatg ggttttgatt
661tttgtgtatc atcaaccaat ccttctgaac aagagcctaa atttccttgt aaagaatggg
721acccaaattt accttcattg tgtcttccaa atcctgaata tttggctcct gaatacatac
781tttctgtgag ctgtgaaaca gccagtgata tgtattcttt aggaactgtt atgtatgctg
841tatttaataa agggaaacct atatttgaag tcaacaagca agatatttac aagagtttca
901gtaggcagtt ggatcagttg agtcgtttag gatctagttc acttacaaat atacctgagg
961aagttcgtga acatgtaaag ctactgttaa atgtaactcc gactgtaaga ccagatgcag
1021atcaaatgac aaagattccc ttctttgatg atgttggtgc agtaacactg caatattttg
1081ataccttatt ccaaagagat aatcttcaga aatcacagtt tttcaaagga ctgccaaagg
1141ttctaccaaa actgcccaag cgtgtcattg tgcagagaat tttgccttgt ttgacttcag
1201aatttgtaaa ccctgacatg gtaccttttg ttttgcccaa tgttctactt attgctgagg
1261aatgcaccaa agaagaatat gtcaaattaa ttcttcctga acttggccct gtgtttaagc
1321agcaggagcc aatccagatt ttgttaattt tcctacaaaa aatggatttg ctactaacca
1381aaacccctcc tgatgagata aagaacagtg ttctacccat ggtttacaga gcactagaag
1441ctccttccat tcagatccag gagctctgtc taaacatcat tccaaccttt gcaaatctta
1501tagactaccc atccatgaaa aacgctttga taccaagaat taaaaatgct tgtctacaaa
1561catcttccct tgcggttcgt gtaaattcat tagtgtgctt aggaaagatt ttggaatact
1621tggataagtg gtttgtactt gatgatatcc tacccttctt acaacaaatt ccatccaagg
1681aacctgcggt cctcatggga attttaggta tttacaaatg tacttttact cataagaagt
1741tgggaatcac caaagagcag ctggccggaa aagtgttgcc tcatcttatt cccctgagta
1801ttgaaaacaa tcttaatctt aatcagttca attctttcat ttccgtcata aaagaaatgc
1861ttaatagatt ggagtctgaa cataagacta aactggagca acttcatata atgcaagaac
1921agcagaaatc tttggatata ggaaatcaaa tgaatgtttc tgaggagatg aaagttacaa
1981atattgggaa tcagcaaatt gacaaagttt ttaacaacat tggagcagac cttctgactg
2041gcagtgagtc cgaaaataaa gaggacgggt tacagaataa acataaaaga gcatcactta
2101cacttgaaga aaaacaaaaa ttagcaaaag aacaagagca ggcacagaag ctgaaaagcc
2161agcagcctct taaaccccaa gtgcacacac ctgttgctac tgttaaacag actaaggact
2221tgacagacac actgatggat aatatgtcat ccttgaccag cctttctgtt agtaccccta
2281aatcttctgc ttcaagtact ttcacttctg ttccttccat gggcattggt atgatgtttt
2341ctacaccaac tgataataca aagagaaatt tgacaaatgg cctaaatgcc aatatgggct
2401ttcagacttc aggattcaac atgcccgtta atacaaacca gaacttctac agtagtccaa
2461gcacagttgg agtgaccaag atgactctgg gaacacctcc cactttgcca aacttcaatg
2521ctttgagtgt tcctcctgct ggtgcaaagc agacccaaca aagacccaca gatatgtctg
2581cccttaataa tctctttggc cctcagaaac ccaaagttag catgaaccag ttatcacaac
2641agaaaccaaa tcagtggctt aatcagtttg tacctcctca aggttctcca actatgggca
2701gttcagtaat ggggacacag atgaacgtga taggacaatc tgcttttggt atgcagggta
2761atcctttctt taacccacag aactttgcac agccaccaac tactatgacc aatagcagtt
2821cagctagcaa tgatttaaaa gatctttttg ggtgcccaac tttcttgtac aaagtggttg
2881gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
2941accggtggcg cgttaagtcg acaatcaacc tctggatta

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