Construct: ORF ccsbBroad304_03666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002751.1_s304c1, BRDN0000402630
- Derived from:
- ccsbBroadEn_03666
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CISD1 (55847)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03666
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55847 | CISD1 | CDGSH iron sulfur domain 1 | NM_018464.5 | 100% | 100% | |
| 2 | mouse | 52637 | Cisd1 | CDGSH iron sulfur domain 1 | NM_134007.4 | 81.5% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 390
- ORF length:
- 324
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tctgacttcc agttccagcg tacgagttga atggatcgca gcagttacca 121 ttgctgctgg gacagctgca attggttatc tagcttacaa aagattttat gttaaagatc 181 atcgaaataa agctatgata aaccttcaca tccagaaaga caaccccaag atagtacatg 241 cttttgacat ggaggatttg ggagataaag ctgtgtactg ccgttgttgg aggtccaaaa 301 agttcccatt ctgtgatggg gctcacacaa aacataacga agagactgga gacaatgtgg 361 gccctctgat catcaagaaa aaagaaactt acccaacttt cttgtacaaa gtggttggta 421 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 481 ggtggcgcgt taagtcgaca atcaacctct ggatta