Construct: ORF ccsbBroad304_03698

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010954.1_s304c1, BRDN0000408346
Derived from:
ccsbBroadEn_03698
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCDHGC4 (56098)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_03698
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56098 PCDHGC4 protocadherin gamma subfami... NM_032406.1 100% 100%
2 human 56098 PCDHGC4 protocadherin gamma subfami... NM_018928.2 89.6% 88.3% (many diffs)
3 mouse 93707 Pcdhgc4 protocadherin gamma subfami... NM_033582.2 82.6% 84.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2682
ORF length:
2613
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gctccgcaag gtgagaagct ggacagaaat ctggcggtgg gctacccttt
121tgttcctctt ttaccacctg ggttacgttt gtgggcagat ccgctacccg gtcccagagg
181agtcacagga agggactttt gtagggaatg tcgctcaaga tttcctgctg gatacggaca
241gtctgtcagc tcgcaggctg caggtcgctg gagaggtgaa ccaaagacac ttccgtgtgg
301atttggacag cggagccctg ctcatcaaga acccaatcga ccgagaggca ctgtgtgggc
361tcagtgccag ctgcatcgtg cccctggagt ttgtcaccga aggtcctttg gaaatgtacc
421gagcagaggt agagatcgta gatgtgaatg atcacgcccc ccgttttccg cggcagcagc
481tggacttgga aattggggag gcagctcctc caggacagcg tttcccgttg gaaaaggctc
541aggatgcaga tgtggggagc aattcgatta gcagctatag gctgagctcc aatgaacact
601ttgcactgga tgtgaagaag cgcagcgacg gcagcctggt cccagagctg ctcctggaga
661agcctttgga tcgagagaag caatcggact accgcctggt gctgactgct gtcgatggag
721ggaacccgcc gagatctggc accgcagagc tccgggtatc cgtgctggac gtaaacgaca
781acgccccagc cttccagcaa tccagctaca ggattagtgt gttggagagc gcaccagcgg
841gcatggtgct catccagctc aatgcctcag acccggacct gggtcccagt ggtaacgtca
901ccttttattt cagtggtcat acccctgatc gtgtaagaaa cctctttagc ctgcacccca
961ctactggaaa gcttactctt ttggggcccc tagactttga gagtgagaat tactatgaat
1021ttgatgtgcg ggctcgcgat gggggttctc cagccatgga gcaacattgc agccttcgag
1081tggatctgct ggacgtaaat gacaatgccc cttacatcac agtgacctca gagcttggaa
1141ccctccccga gagtgcagaa cctggcactg tggtggcact tatcagtgtg caggatccag
1201actcagggtc aaacggagat gtgagcctcc gcattcctga ccacttgcca tttgccctca
1261agtctgcctt caggaaccag ttctccctgg tgactgctgg acccttggat cgagaggcca
1321aatctagcta tgacatcatg gtcactgctt ctgatgctgg gaaccctcct ctcagtaccc
1381acagaactat tttcctcaat atttcagatg tgaatgataa tccaccctct ttctttcaga
1441ggtcacatga ggtgtttgtt cctgagaaca atcgcccagg ggacctgctt tgctcccttg
1501cagcctctga cccagactct ggcttgaatg cgcttatctc ctactcactc ctggagccca
1561ggaatcgaga tgtatcagct tcctctttca tctctctgaa cccccagaca ggagctgttc
1621atgctactcg atcctttgac tatgagcaaa cccagacact gcagtttgag gtgcaggccc
1681gggatcgggg caacccaccc cttagcagca ctgtaacagt tcgtctattt gtgctggacc
1741tcaatgacaa tgctccagct gtgctccgtc ctcgggcccg gcctggttcc ttatgtcccc
1801aagcactgcc tccatcagtt ggtgctggcc acctaatcac aaaggtgact gctgtggact
1861tggattcagg ttacaatgct tgggtttcct atcagctcct ggaggcccca gatcccagcc
1921tgtttgcagt ctctcgatat gctggggagg tgcggacggc tgttcctatc ccagctgacc
1981tcccaccaca gaagctggtc attgtggtaa aggatagtgg tagtccacca ctctctacct
2041ctgttactct cttagtgtcc ttagaggaag acactcatcc agttgtccca gatcttcgag
2101aatcttcagc tccaagggaa ggagaatctc gtctaaccct ctacttggct gtgtccctag
2161tggcaatttg ctttgtctcc tttggctcat tcgtggcact actctctaag tgtcttcgtg
2221gggcagcctg tggagtcaca tgctttcctg ctggcacctg tgcctgtctc accagatctc
2281gaaggaggga ggggcttccc ccttccaatg ggatcctccg aatccagcta gggtcagatg
2341accctatcaa gtttgttgat gtgggaggcc actctcatgg ctgtacaccc ttggcttctg
2401cacccactcg gagtgatagc ttcatgatgg tgaagtcacc cagtgcacct atggcagggg
2461agcctgttcg cccaagctgc ccaccctctg atcttctcta tgggctagag gtgagacctt
2521tgcaggctca acaaatgctt gagggttatt ctgatccagg catatggcta ggccatgtcc
2581tagagagtac tggcctctca gtaagtgccc atagtgatgt caccattttt gtaagaggta
2641actatgtggt agacgctgtg ctttgtaact gtttcgtgaa tttgccaact ttcttgtaca
2701aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2761ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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