Construct: ORF ccsbBroad304_03775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009483.1_s304c1, BRDN0000401552
- Derived from:
- ccsbBroadEn_03775
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAZ3 (57054)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03775
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57054 | DAZ3 | deleted in azoospermia 3 | NM_020364.3 | 100% | 100% | |
| 2 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_020420.3 | 88.7% | 88.1% | (many diffs) |
| 3 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005786.2 | 82.9% | 81.9% | (many diffs) |
| 4 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005785.2 | 81% | 80.3% | (many diffs) |
| 5 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_020363.3 | 77.6% | 77% | (many diffs) |
| 6 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531483.1 | 74.2% | 73.7% | (many diffs) |
| 7 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_011531510.1 | 69.1% | 68.8% | (many diffs) |
| 8 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_001005375.2 | 68.1% | 67.6% | (many diffs) |
| 9 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_011531509.3 | 64.7% | 64.3% | (many diffs) |
| 10 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_005262602.1 | 60.2% | 59.8% | (many diffs) |
| 11 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531482.2 | 57.2% | 56.8% | (many diffs) |
| 12 | human | 1617 | DAZ1 | deleted in azoospermia 1 | NM_004081.5 | 53.4% | 53.1% | (many diffs) |
| 13 | human | 1618 | DAZL | deleted in azoospermia like | NM_001351.4 | 50.8% | 38.2% | (many diffs) |
| 14 | human | 1618 | DAZL | deleted in azoospermia like | NM_001190811.1 | 48.7% | 36.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1383
- ORF length:
- 1314
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgctgca aatcctgaga ctccaaactc aaccatctcc agagaggcca 121 gcacccagtc ttcatcagct gcagctagcc aaggctgggt gttaccagaa ggcaaaatcg 181 tgccaaacac tgtttttgtt ggtggaattg atgctaggat ggatgaaact gagattggaa 241 gctgctttgg tagatacggt tcagtgaaag aagtgaagat aatcacgaat cgaactggtg 301 tgtccaaagg ctatggattt gtttcgtttg ttaatgacgt ggatgtccag aagatagtag 361 gatcacagat acatttccat ggtaaaaagc tgaagctggg ccctgcaatc aggaaacaaa 421 agttatgtgc tcgtcatgtg cagccacgtc ctttggtagt taatcctcct cctccaccac 481 agtttcagaa cgtctggcgg aatccaaaca ctgaaaccta cctgcagccc caaatcacgc 541 cgaatcctgt aactcagcac gttcaggctt actctgctta tccacattca ccaggtcagg 601 tcatcactgg atgtcagttg cttgtatata attatcagga atatcctact tatcccgatt 661 cagcatttca ggtcaccact ggatatcagt tgcctgtata taattatcag ccatttcctg 721 cttatccaag atcaccattt caggtcactg ctggatatca gttgcctgta tataattatc 781 aggcatttcc tgcttatcca aattcaccat ttcaagtcgc cactggatat cagttccctg 841 tatacaatta tcaggcattt cctgcttatc caaattcacc agttcaggtc accactggat 901 atcagttgcc tgtatacaat tatcaggcat ttcctgctta tccaaattca ccatttcaag 961 tcgccactgg atatcagttc cctgtataca attatcaggc atttcctgct tatccaaatt 1021 caccagttca ggtcaccact ggatatcagt tgcctgtata caattatcag gcatttcctg 1081 cttatccaaa ttcaccattt caagtcgcca ctggatatca gttccctgta tacaattatc 1141 aggcatttcc tgcttatcca aattcaccag ttcaggtcac cactggatat cagttgcctg 1201 tatacaatta tcaggcattt cctgcttatc caaattcagc agttcaggtc accactggat 1261 atcagttcca tgtatacaat taccagatgc caccgcagtg ccctgttggg gagcaaagga 1321 gaaatctgtg gaccgaagca tacaaatggt ggtatcttgt ctgtttaatc cagagaagag 1381 acttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1441 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1501 tctggatta