Transcript: Human NM_020420.3

Homo sapiens deleted in azoospermia 4 (DAZ4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-18
Taxon:
Homo sapiens (human)
Gene:
DAZ4 (57135)
Length:
3388
CDS:
308..1480

Additional Resources:

NCBI RefSeq record:
NM_020420.3
NBCI Gene record:
DAZ4 (57135)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020420.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000428871 CAGTTCCCTGTATACAATTAT pLKO_005 1070 CDS 100% 15.000 7.500 Y DAZ3 n/a
2 TRCN0000430230 CAGTTGCCTGTATACAATTAT pLKO_005 1286 CDS 100% 15.000 7.500 Y DAZ3 n/a
3 TRCN0000428349 AGTTGCCTGTATACAATTATC pLKO_005 1287 CDS 100% 13.200 6.600 Y DAZ2 n/a
4 TRCN0000423819 CACGTCCTTTGGTAGTTAATC pLKO_005 684 CDS 100% 13.200 6.600 Y DAZ3 n/a
5 TRCN0000083758 CCACGTCCTTTGGTAGTTAAT pLKO.1 683 CDS 100% 13.200 6.600 Y DAZ2 n/a
6 TRCN0000154085 CCACGTCCTTTGGTAGTTAAT pLKO.1 683 CDS 100% 13.200 6.600 Y DAZ4 n/a
7 TRCN0000435893 CTGATTTGAAAGCTAACAATT pLKO_005 1905 3UTR 100% 13.200 6.600 Y DAZ3 n/a
8 TRCN0000431144 GCCACGTCCTTTGGTAGTTAA pLKO_005 682 CDS 100% 13.200 6.600 Y DAZ1 n/a
9 TRCN0000434665 ACTGATAAATTCCGTTGTTAC pLKO_005 1476 CDS 100% 10.800 5.400 Y DAZ1 n/a
10 TRCN0000418585 CTTATCCAAGATCACCATTTC pLKO_005 960 CDS 100% 10.800 5.400 Y DAZ1 n/a
11 TRCN0000153130 GCACCATGACTTTGTCCATTA pLKO.1 3038 3UTR 100% 10.800 5.400 Y DAZ3 n/a
12 TRCN0000416803 GCATTTCCTGCTTATCCAAAT pLKO_005 1022 CDS 100% 10.800 5.400 Y DAZ2 n/a
13 TRCN0000083760 GCTTATCCAAATTCACCATTT pLKO.1 1031 CDS 100% 10.800 5.400 Y DAZ2 n/a
14 TRCN0000150791 GCTTATCCAAGATCACCATTT pLKO.1 959 CDS 100% 10.800 5.400 Y DAZ4 n/a
15 TRCN0000150581 GCTTATCCAAGTTCACCATTT pLKO.1 1103 CDS 100% 10.800 5.400 Y DAZ4 n/a
16 TRCN0000156768 GTTCAGGTCACCACTGGATAT pLKO.1 1265 CDS 100% 10.800 5.400 Y DAZ3 n/a
17 TRCN0000083761 CCAAAGGCTATGGATTTGTTT pLKO.1 543 CDS 100% 5.625 2.813 Y DAZ2 n/a
18 TRCN0000150990 CCAAAGGCTATGGATTTGTTT pLKO.1 543 CDS 100% 5.625 2.813 Y DAZ4 n/a
19 TRCN0000119017 CCTGATTTGAAAGCTAACAAT pLKO.1 1904 3UTR 100% 5.625 2.813 Y DAZ1 n/a
20 TRCN0000157422 GACGTGGATGTCCAGAAGATA pLKO.1 575 CDS 100% 5.625 2.813 Y DAZ4 n/a
21 TRCN0000151232 GCATACAAATGGTGGTATCTT pLKO.1 1433 CDS 100% 5.625 2.813 Y DAZ4 n/a
22 TRCN0000119018 GCCCTGCAATCAGGAAACAAA pLKO.1 639 CDS 100% 5.625 2.813 Y DAZ1 n/a
23 TRCN0000157556 GCCCTGCAATCAGGAAACAAA pLKO.1 639 CDS 100% 5.625 2.813 Y DAZ4 n/a
24 TRCN0000154176 CCCTTCTTTCAATGGCTTCTT pLKO.1 2854 3UTR 100% 4.950 2.475 Y DAZ3 n/a
25 TRCN0000152171 CCTTCTCAATGAGTTACCATT pLKO.1 2299 3UTR 100% 4.950 2.475 Y DAZ3 n/a
26 TRCN0000119019 CGGTTCAGTGAAAGAAGTGAA pLKO.1 496 CDS 100% 4.950 2.475 Y DAZ1 n/a
27 TRCN0000153902 CGGTTCAGTGAAAGAAGTGAA pLKO.1 496 CDS 100% 4.950 2.475 Y DAZ4 n/a
28 TRCN0000158257 CGTTCAGGCTTACTCTGCTTA pLKO.1 799 CDS 100% 4.950 2.475 Y DAZ4 n/a
29 TRCN0000150631 GAAGATAGTAGGATCACAGAT pLKO.1 589 CDS 100% 4.950 2.475 Y DAZ3 n/a
30 TRCN0000119020 GCTGGATATCAGTTGCCTGTA pLKO.1 989 CDS 100% 4.050 2.025 Y DAZ1 n/a
31 TRCN0000157003 GCTGGATATCAGTTGCCTGTA pLKO.1 989 CDS 100% 4.050 2.025 Y DAZ4 n/a
32 TRCN0000151309 GCTTATCCAAATTCACCAGTT pLKO.1 1247 CDS 100% 4.050 2.025 Y DAZ4 n/a
33 TRCN0000083762 GCTTATCCAAATTCAGCAGTT pLKO.1 1319 CDS 100% 4.050 2.025 Y DAZ2 n/a
34 TRCN0000151386 GCTTATCCAAATTCAGCAGTT pLKO.1 1319 CDS 100% 4.050 2.025 Y DAZ4 n/a
35 TRCN0000152311 CACAGATACATTTCCATGGTA pLKO.1 603 CDS 100% 3.000 1.500 Y DAZ3 n/a
36 TRCN0000150746 GAATCCAAACACTGAAACCTA pLKO.1 739 CDS 100% 3.000 1.500 Y DAZ4 n/a
37 TRCN0000083759 CGAAGCATACAAATGGTGGTA pLKO.1 1429 CDS 100% 2.640 1.320 Y DAZ2 n/a
38 TRCN0000153930 CGAAGCATACAAATGGTGGTA pLKO.1 1429 CDS 100% 2.640 1.320 Y DAZ4 n/a
39 TRCN0000156515 GCTGCTTTGGTAGATACGGTT pLKO.1 480 CDS 100% 2.640 1.320 Y DAZ4 n/a
40 TRCN0000157295 CCGAAGCATACAAATGGTGGT pLKO.1 1428 CDS 100% 2.160 1.080 Y DAZ3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020420.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03792 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03792 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000492087 ACCTACCTTCTCGAATATTAGTAA pLX_317 32.6% 100% 100% V5 n/a
4 ccsbBroadEn_03775 pDONR223 100% 88.7% 88.1% None (many diffs) n/a
5 ccsbBroad304_03775 pLX_304 0% 88.7% 88.1% V5 (many diffs) n/a
6 TRCN0000469955 GAACCATTAACTAACTAATCCAAA pLX_317 30.9% 88.7% 88.1% V5 (many diffs) n/a
7 ccsbBroadEn_08693 pDONR223 100% 72.9% 73% None 504C>T;993_994ins432 n/a
8 ccsbBroad304_08693 pLX_304 0% 72.9% 73% V5 504C>T;993_994ins432 n/a
9 TRCN0000472354 CTGAGTTACTATTCACGCCTCTGT pLX_317 4.1% 72.9% 73% V5 504C>T;993_994ins432 n/a
10 ccsbBroadEn_14611 pDONR223 73.1% 37.5% 35.5% None (many diffs) n/a
11 ccsbBroad304_14611 pLX_304 0% 37.5% 35.5% V5 (not translated due to frame shift) (many diffs) n/a
12 TRCN0000491388 ACATGCTGGCTAGCAGTGCCTTTA pLX_317 18.9% 37.5% 35.5% V5 (not translated due to frame shift) (many diffs) n/a
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