Construct: ORF ccsbBroad304_03826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011570.1_s304c1, BRDN0000462096
- Derived from:
- ccsbBroadEn_03826
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRFN2 (57497)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03826
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57497 | LRFN2 | leucine rich repeat and fib... | NM_020737.3 | 100% | 100% | |
| 2 | human | 57497 | LRFN2 | leucine rich repeat and fib... | XM_011514761.2 | 100% | 100% | |
| 3 | human | 57497 | LRFN2 | leucine rich repeat and fib... | XM_011514762.2 | 100% | 100% | |
| 4 | human | 57497 | LRFN2 | leucine rich repeat and fib... | XM_017011110.1 | 100% | 100% | |
| 5 | human | 57497 | LRFN2 | leucine rich repeat and fib... | XM_017011111.1 | 59.1% | 59.1% | 1401_1402ins966 |
| 6 | mouse | 70530 | Lrfn2 | leucine rich repeat and fib... | NM_027452.3 | 86.7% | 95.1% | (many diffs) |
| 7 | mouse | 70530 | Lrfn2 | leucine rich repeat and fib... | XM_006524896.2 | 54.1% | 58% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2436
- ORF length:
- 2367
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaccctg cttggtggcc tgctagcgtt tggcatggcg tttgccgtgg 121 tcgacgcctg ccccaagtac tgtgtctgcc agaatctgtc tgagtcactg gggaccctgt 181 gcccctccaa ggggctgctc tttgtacccc ctgatattga ccggcggaca gtggagctgc 241 gcctgggcgg caacttcatc atccacatca gccgccagga ctttgccaac atgacggggc 301 tggtggacct gaccctgtcc aggaacacca tcagccacat ccagcccttt tcctttctgg 361 acctcgagag cctccgctcc ctgcatcttg acagcaatcg gctgccaagc cttggggagg 421 acaccctccg gggcctggtc aacctgcagc accttatcgt gaacaacaac cagctgggcg 481 gcatcgcaga tgaggctttt gaggacttcc tgctgacatt ggaggatctg gacctctcct 541 acaacaacct ccatggcctg ccgtgggact ccgtgcgacg catggtcaac ctccaccagc 601 tgagcctgga ccacaacctg ctggatcaca tcgccgaggg cacctttgca gacctgcaga 661 aactggcccg cctggatctc acctccaatc ggctgcagaa gctgccccct gatcccatct 721 ttgcccgctc ccaggcttcg gctttgacag ccacaccctt tgccccaccc ttgtccttta 781 gttttggggg taacccactt cactgcaatt gtgagcttct ctggctgcgg aggctcgagc 841 gggacgatga cctggaaacc tgtggctccc cagggggcct caagggtcgc tacttctggc 901 atgtgcgtga ggaggagttt gtgtgcgagc cgcctctcat cacccagcac acacacaagt 961 tgctggttct ggagggccag gcggccacac tcaagtgcaa agccattggg gaccccagcc 1021 cccttatcca ctgggtagcc cccgatgacc gcctggtagg gaactcctca aggaccgctg 1081 tctatgacaa tggcaccctg gacatcttca tcaccacatc tcaggacagt ggtgccttca 1141 cctgcattgc tgccaatgct gccggagagg ccacggccat ggtggaggtc tccatcgtcc 1201 agctgccaca cctcagcaac agcaccagcc gcactgcacc ccccaagtcc cgcctctcag 1261 acatcactgg ctccagcaag accagccggg gaggtggagg cagtgggggc ggagagcctc 1321 ccaaaagccc cccggaacgg gctgtgcttg tgtctgaagt gaccaccacc tcggccctgg 1381 tcaagtggtc tgtcagcaag tcagcacccc gggtgaagat gtaccagctg cagtacaact 1441 gctctgacga tgaggtactg atttacagga tgatcccagc ctccaacaag gccttcgtgg 1501 tcaacaacct ggtgtcaggg actggctacg acttgtgtgt gctggccatg tgggatgaca 1561 cagccacgac actcacggcc accaacatcg tgggctgcgc ccagttcttc accaaggctg 1621 actacccgca gtgccagtcc atgcacagcc agattctggg cggcaccatg atcctggtca 1681 tcgggggcat catcgtggcc acgctgctgg tcttcatcgt catcctcatg gtgcgctaca 1741 aggtctgcaa ccacgaggcc cccagcaaga tggcagcggc cgtgagcaat gtgtactcgc 1801 agaccaacgg cgcccagcca ccgcctccaa gcagcgcacc agccggggcc ccgccgcagg 1861 gcccgccgaa ggtggtggtg cgcaacgagc tcctggactt caccgccagc ctggcccgcg 1921 ccagtgactc ctcttcctcc agctccctgg gcagtgggga ggctgcgggg ctgggacggg 1981 ccccctggag gatcccaccc tccgccccgc gccccaagcc cagccttgac cgcctgatgg 2041 gggccttcgc ctccctggac ctcaagagtc agagaaagga ggagctgctg gactccagga 2101 ctccagccgg gagaggggct gggacgtcgg cccggggcca ccactcggac cgagagccac 2161 tgctggggcc ccctgcggcc cgggccagga gcctgctccc cttgccgttg gagggcaagg 2221 ccaaacgcag ccactccttc gacatggggg actttgctgc tgcggcggcg ggaggggtcg 2281 tgccgggcgg ctacagtcct cctcggaagg tctcgaacat ctggacgaag cgcagcctct 2341 ctgtcaacgg catgctcttg ccctttgagg agagtgacct ggtgggggcc cgggggactt 2401 ttggcagctc cgaatgggtg atggagagca cggtcttgcc aactttcttg tacaaagtgg 2461 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2521 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta