Construct: ORF ccsbBroad304_03895
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010335.1_s304c1, BRDN0000408027
- Derived from:
- ccsbBroadEn_03895
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCOC (60592)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03895
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60592 | SCOC | short coiled-coil protein | NM_001153635.1 | 100% | 100% | |
| 2 | human | 60592 | SCOC | short coiled-coil protein | NM_001153446.1 | 99.1% | 99.1% | 142_144delGCA |
| 3 | human | 60592 | SCOC | short coiled-coil protein | NM_001153585.1 | 99.1% | 99.1% | 142_144delGCA |
| 4 | human | 60592 | SCOC | short coiled-coil protein | NM_032547.3 | 99.1% | 99.1% | 142_144delGCA |
| 5 | human | 60592 | SCOC | short coiled-coil protein | NM_001153690.1 | 77.6% | 77.6% | 141_142ins81 |
| 6 | human | 60592 | SCOC | short coiled-coil protein | NM_001153663.1 | 67.6% | 64.3% | (many diffs) |
| 7 | human | 60592 | SCOC | short coiled-coil protein | NM_001153484.1 | 67.2% | 63.9% | (many diffs) |
| 8 | human | 60592 | SCOC | short coiled-coil protein | NM_001153552.1 | 51% | 47.5% | (many diffs) |
| 9 | mouse | 56367 | Scoc | short coiled-coil protein | XM_011248448.3 | 86.5% | 85.4% | (many diffs) |
| 10 | mouse | 56367 | Scoc | short coiled-coil protein | NM_001039137.3 | 85.8% | 84.8% | (many diffs) |
| 11 | mouse | 56367 | Scoc | short coiled-coil protein | NM_001285992.1 | 62.5% | 66.9% | (many diffs) |
| 12 | mouse | 56367 | Scoc | short coiled-coil protein | XM_006531205.2 | 62.5% | 66.9% | (many diffs) |
| 13 | mouse | 56367 | Scoc | short coiled-coil protein | NM_019708.4 | 62% | 66.3% | (many diffs) |
| 14 | mouse | 56367 | Scoc | short coiled-coil protein | XM_006531204.4 | 62% | 66.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 429
- ORF length:
- 363
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgggtccagg aaagaggagg aggaagacag cacattcacc aacatttctc 121 ttgcagatga catagaccat tcctcaagaa ttttgtatcc aaggcccaaa agtttgttac 181 ccaagatgat gaatgctgac atggatgttg atgctgaaaa tcaagtggaa ctggaggaaa 241 aaacaagact tattaatcaa gtgttggaac tccaacacac acttgaagat ctctctgcaa 301 gagtagatgc agttaaggaa gaaaatctga agctaaaatc agaaaaccaa gttcttggac 361 aatatataga aaatctcatg tcagcttcta gtgtttttca aacaactgac acaaaaagca 421 aaagaaagta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 481 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 541 tcaacctctg gatta