Construct: ORF ccsbBroad304_03939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001132.1_s304c1, BRDN0000403562
- Derived from:
- ccsbBroadEn_03939
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF25 (64320)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03939
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64320 | RNF25 | ring finger protein 25 | NM_022453.3 | 100% | 100% | |
| 2 | human | 64320 | RNF25 | ring finger protein 25 | XM_017004695.2 | 75.5% | 75.5% | 0_1ins336 |
| 3 | mouse | 57751 | Rnf25 | ring finger protein 25 | NM_021313.3 | 84.4% | 82.3% | (many diffs) |
| 4 | mouse | 57751 | Rnf25 | ring finger protein 25 | NM_001305230.1 | 84.2% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1443
- ORF length:
- 1377
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtctgcg tctgcagctg caggggagga ggactgggtc cttccctctg 121 aagttgaagt attggagtcc atctatctag atgaactaca ggtgattaaa ggaaatggca 181 gaacttcacc atgggagatc tacatcactt tgcatcctgc cactgcagag gaccaggatt 241 cacagtatgt ctgcttcact ctggtgcttc aggtcccagc agagtatccc catgaggtgc 301 cacagatctc tatccgaaat ccccgaggac tttcagatga acagatccac acgatcttac 361 aggtgctggg ccacgtggcc aaggctgggc tgggcactgc catgctgtat gaactcattg 421 agaaagggaa ggaaattctc acagataaca acatccctca tggccagtgt gtcatctgcc 481 tctatggttt ccaggagaag gaggccttta ccaaaacacc ctgttaccac tacttccact 541 gccactgcct tgctcggtac atccagcaca tggagcaaga gctgaaggca caaggacagg 601 agcaggaaca ggaacggcag catgctacaa ccaaacagaa ggcagtcggt gtgcagtgtc 661 cagtgtgcag agagcccctc gtgtatgatc ttgcctcact gaaagcagcc cctgaacccc 721 aacagcccat ggagctgtac cagcccagtg cagagagctt gcgccagcaa gaagaacgca 781 agcggctcta ccagaggcag caggagcggg ggggaatcat tgaccttgag gctgagcgaa 841 accgatactt catcagcctt cagcagcctc ctgcccctgc ggaacctgag tcagctgtag 901 atgtctccaa aggatcccaa ccacccagca cccttgcagc agaactatcc acctcaccag 961 ccgtccaatc cactttgcca cctcctctgc ctgtggcgac ccagcacata tgtgagaaga 1021 ttccagggac caggtcaaat cagcaaaggt tgggcgaaac ccagaaagct atgctagatc 1081 cccccaagcc cagtcgaggt ccctggcgac agcccgaacg gaggcaccca aagggagggg 1141 agtgccacgc ccctaaaggt acccgtgaca cccaggaact gccacctcct gaggggcccc 1201 tcaaggagcc catggaccta aagccagaac cccatagcca aggagttgaa ggtcctccac 1261 aagagaaggg gcctggcagc tggcaggggc ccccaccccg caggactcgg gactgtgttc 1321 gctgggagcg ctctaaaggc cggacacccg gttcttccta ccctcgcctg cctcggggcc 1381 agggagcata ccggcctggt actcggaggg agtccctggg cctggaatct aaggatggtt 1441 cctacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1501 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1561 tctggatta