Construct: ORF ccsbBroad304_03987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001899.1_s304c1, BRDN0000402277
- Derived from:
- ccsbBroadEn_03987
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPH (64946)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03987
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64946 | CENPH | centromere protein H | NM_022909.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagcagccc cagatgcaag acgccgacga gcccgcggac tccggagggg 121 aaggccgggc aggcgggcca ccgcaggtcg ccggcgccca ggcggcgtgc agcgaggacc 181 gcatgaccct gctcctcagg ctgagagcac agacaaaaca acaactctta gaatataaat 241 caatggttga tgcaagtgaa gaaaaaactc cagaacaaat tatgcaagaa aagcaaatcg 301 aagctaaaat tgaagacctg gaaaatgaaa ttgaagaggt aaaagttgct tttgagataa 361 aaaagcttgc attagacagg atgagacttt caactgcact taaaaaaaac ctggagaaaa 421 ttagcagaca gtctagtgtg ctcatggata acatgaaaca cctattagag ctaaataaat 481 taataatgaa atcacagcag gaatcttggg atttagagga aaaactgctt gatattagaa 541 agaagagatt gcaattaaaa caagcttcag aaagtaagct tttagaaata cagactgaaa 601 agaacaaaca gaagattgat ttggacagta tggaaaactc agagaggata aagatcatac 661 gacaaaacct acagatggag ataaaaatta ctactgttat tcaacatgtg ttccagaacc 721 ttattttggg gagtaaagtc aattgggcag aggatcctgc ccttaaggaa attgttctgc 781 agcttgagaa gaatgttgac atgatgtacc caactttctt gtacaaagtg gttggtaagc 841 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 901 ggcgcgttaa gtcgacaatc aacctctgga tta