Construct: ORF ccsbBroad304_04017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016948.1_s304c1, BRDN0000409606
- Derived from:
- ccsbBroadEn_04017
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRFN4 (78999)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04017
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78999 | LRFN4 | leucine rich repeat and fib... | NM_001363524.2 | 100% | 100% | |
| 2 | human | 78999 | LRFN4 | leucine rich repeat and fib... | NM_024036.5 | 100% | 100% | |
| 3 | mouse | 225875 | Lrfn4 | leucine rich repeat and fib... | NM_153388.4 | 87.7% | 96.6% | (many diffs) |
| 4 | mouse | 225875 | Lrfn4 | leucine rich repeat and fib... | XM_017318142.1 | 87.7% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1971
- ORF length:
- 1905
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgacatggc cccgccgctc ctgctgctgc tgctggccag tggagcggcc gcctgcccgc 121 tgccctgcgt ctgccagaac ctgtccgagt cgctcagcac cctctgtgcc caccgaggcc 181 tgctgtttgt gccgcccaac gtggaccggc gcacagtgga gctgcggctg gctgacaact 241 tcatccaggc cctggggccc cctgacttcc gcaacatgac gggactggtg gacctgacac 301 tgtctcgcaa tgccatcacc cgcattgggg cccgcgcctt tggggacctc gagagcctgc 361 gttccctcca ccttgacggc aacaggctgg tggagctggg caccgggagc ctccggggcc 421 ccgtcaatct gcagcacctc atcctcagcg gcaaccagct gggccgcatc gcgccgggag 481 ccttcgacga cttcctagag agcctggagg acctggacct gtcctacaac aacctccggc 541 aggtgccctg ggccggcatc ggcgccatgc ctgccctgca caccctcaac ctggaccata 601 accttattga cgcactgccc ccaggcgcct tcgcccagct cggtcagctc tcccgcctgg 661 acctcacctc caaccgcctg gccacgctgg ctccggaccc gcttttctct cgtgggcgtg 721 atgcagaggc ctctcccgcc cccctggtgc tgagctttag cgggaacccc ctgcactgca 781 actgtgagct gctgtggctg cggcggctgg cgcggccgga cgacctggaa acgtgcgcct 841 ccccgcccgg cctggccggc cgctacttct gggcagtgcc cgagggcgag ttctcctgtg 901 agccgcccct cattgcccgc cacacgcagc gcctctgggt gctggaaggc cagcgggcca 961 cgctgcggtg ccgggccctg ggtgaccccg cgcctaccat gcactgggtc ggtcctgacg 1021 accggttggt tggcaactcc tcccgagccc gggctttccc caacgggacc ttagagattg 1081 gggtgaccgg cgctggggac gctgggggct acacctgcat cgccaccaac cctgctggtg 1141 aggccacagc ccgagtagaa ctgcgggtgc tggccttgcc ccatggtggg aacagcagtg 1201 ccgagggggg ccgccccggg ccctcggaca tcgccgcctc cgctcgcact gctgccgagg 1261 gtgaggggac gctggagtct gagccagccg tgcaggtgac ggaggtgacc gccacctcag 1321 ggctggtgag ctggggtccc gggcggccag ccgacccagt gtggatgttc caaatccagt 1381 acaacagcag cgaagatgag accctcatct accggattgt cccagcctcc agccaccact 1441 tcctgctgaa gcacctcgtc cccggcgctg actatgacct ctgcctgctg gccttgtcac 1501 cggccgctgg gccctctgac ctcacggcca ccaggctgct gggctgtgcc catttctcca 1561 cgctgccggc ctcgcccctg tgccacgccc tgcaggccca cgtgctgggc gggaccctga 1621 ccgtggccgt ggggggtgtg ctggtggctg ccttactggt cttcactgtg gccttgctgg 1681 ttcggggccg gggggccgga aatggccgcc tccccctcaa gctcagccac gtccagtccc 1741 agaccaatgg aggccccagc cccacaccca aggcccaccc gccgcggagc cccccgcccc 1801 ggccgcagcg cagctgctct ctggacctgg gagatgccgg gtgctacggt tatgccaggc 1861 gcctgggagg agcttgggcc cgacggagcc actctgtgca tggggggctg ctcggggcag 1921 ggtgccgggg ggtaggaggc agcgccgagc ggctggaaga gagtgtggtg tgcccaactt 1981 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2041 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta