Construct: ORF ccsbBroad304_04036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012845.1_s304c1, BRDN0000410933
- Derived from:
- ccsbBroadEn_04036
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRRG4 (79056)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04036
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | NM_024081.6 | 100% | 100% | |
| 2 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_006718314.3 | 100% | 100% | |
| 3 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_017018275.1 | 62% | 62% | 1_414del |
| 4 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_006718313.3 | 41.2% | 29.2% | 1_414del;729_730ins133;864_865ins95 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tacgcttctg gttctactca gccaactgcc cacagttacc ctggggtttc 121 ctcattgcgc aagaggtcca aaggcttcta agcatgcggg agaagaagtg tttacatcaa 181 aagaagaagc aaactttttc atacatagac gccttctgta taatagattt gatctggagc 241 tcttcactcc cggcaaccta gaaagagagt gcaatgaaga actttgcaat tatgaggaag 301 ccagagagat ttttgtggat gaagataaaa cgattgcatt ttggcaggaa tattcagcta 361 aaggaccaac cacaaaatca gatggcaaca gagagaaaat agatgttatg ggccttctga 421 ctggattaat tgctgctgga gtatttttgg ttatttttgg attacttggc tactatcttt 481 gtatcactaa gtgtaatagg ctacaacatc catgctcttc agccgtctat gaaaggggga 541 ggcacactcc ctccatcatt ttcagaagac ctgaggaggc tgccttgtct ccattgccgc 601 cttctgtgga ggatgcagga ttaccttctt atgaacaggc agtggcgctg accagaaaac 661 acagtgtttc accaccacca ccatatcctg ggcacacaaa aggatttagg gtatttaaaa 721 aatctatgtc tctcccatct cactgcccaa ctttcttgta caaagtggtt ggtaagccta 781 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 841 gcgttaagtc gacaatcaac ctctggatta