Transcript: Human XM_006718314.3

PREDICTED: Homo sapiens proline rich and Gla domain 4 (PRRG4), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PRRG4 (79056)
Length:
1123
CDS:
112..792

Additional Resources:

NCBI RefSeq record:
XM_006718314.3
NBCI Gene record:
PRRG4 (79056)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006718314.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000055793 GCAGGATTACCTTCTTATGAA pLKO.1 661 CDS 100% 5.625 7.875 N PRRG4 n/a
2 TRCN0000433135 TGCTCTTCAGCCGTCTATGAA pLKO_005 559 CDS 100% 5.625 7.875 N PRRG4 n/a
3 TRCN0000424146 AGAGGTCCAAAGGCTTCTAAG pLKO_005 178 CDS 100% 10.800 8.640 N PRRG4 n/a
4 TRCN0000055794 GCTACTATCTTTGTATCACTA pLKO.1 515 CDS 100% 4.950 3.960 N PRRG4 n/a
5 TRCN0000055795 GAGAGTGCAATGAAGAACTTT pLKO.1 311 CDS 100% 5.625 3.938 N PRRG4 n/a
6 TRCN0000055797 GAGAAGAAGTGTTTACATCAA pLKO.1 206 CDS 100% 4.950 3.465 N PRRG4 n/a
7 TRCN0000055796 CAGCTAAAGGACCAACCACAA pLKO.1 401 CDS 100% 4.050 2.835 N PRRG4 n/a
8 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 855 3UTR 100% 13.200 6.600 Y LIAS n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006718314.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04036 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04036 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_15978 pDONR223 0% 99.8% 100% None 534G>A n/a
4 ccsbBroad304_15978 pLX_304 0% 99.8% 100% V5 534G>A n/a
5 TRCN0000472222 ACAAGTTCAGGGGCGGGGAACTAG pLX_317 68.7% 99.8% 100% V5 534G>A n/a
Download CSV