Construct: ORF ccsbBroad304_04066

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000173.1_s304c1, BRDN0000409968
Derived from:
ccsbBroadEn_04066
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CENPO (79172)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04066
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79172 CENPO centromere protein O NM_001322101.2 100% 100%
2 human 79172 CENPO centromere protein O NM_024322.3 100% 100%
3 human 79172 CENPO centromere protein O NM_001199803.3 94.1% 93.4% 0_1ins28;6_7insAGTCCAAA;10_27del
4 human 79172 CENPO centromere protein O XR_244957.2 67.4% 1_231del;560_561ins101;994_995ins36
5 human 79172 CENPO centromere protein O NR_136183.2 22.1% (many diffs)
6 human 79172 CENPO centromere protein O NR_136182.2 19.5% (many diffs)
7 human 79172 CENPO centromere protein O NR_136184.2 19.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
966
ORF length:
900
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gcaggcgaac cctttacgtc cagatggcga gtccaaagga ggtgttttag
121ctcacttgga aaggctagag acccaagtga gcagatcccg taaacagtct gaagagctgc
181agagcgtgca ggcccaggaa ggtgctcttg gaaccaagat tcataaacta aggcgtctgc
241gagatgagct gagggctgtg gtgcggcacc ggcgagccag cgtgaaagca tgtattgcca
301atgtagaacc caaccaaaca gtggagatca atgagcaaga agcattggaa gagaaattgg
361aaaatgtgaa agccattctg caggcatatc attttacagg cctcagtggt aaactgacca
421gccgaggagt ttgtgtctgc atcagtactg cttttgaggg gaacctattg gattcctatt
481ttgtggacct tgtcatacag aaaccactcc ggatacatca ccattcagtc ccagtcttca
541ttcccctgga agagatagct gcaaaatatt tacagaccaa catccagcac ttcctgttca
601gtctctgcga gtacctgaat gcttactctg ggaggaagta ccaggcagac cggcttcaga
661gtgactttgc agccctcctg actgggccct tgcagagaaa cccactgtgt aacttgctgt
721catttactta caaactggat ccagggggtc agtccttccc gttctgtgct agattgctgt
781ataaggacct cacagcaact cttcccactg acgtcaccgt gacatgtcaa ggagtggaag
841tattatccac ttcatgggag gagcaacgag catctcatga aactctgttc tgtacgaagc
901ccttgcatca agtgtttgcc tcatttacaa gaaaaggaga aaagttggat atgagtctgg
961tctcctaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1021gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1081acctctggat ta

Download FASTA (ORF) (Full)