Construct: ORF ccsbBroad304_04140

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013627.1_s304c1, BRDN0000406928
Derived from:
ccsbBroadEn_04140
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TCTN2 (79867)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04140
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79867 TCTN2 tectonic family member 2 NM_024809.5 100% 100%
2 human 79867 TCTN2 tectonic family member 2 NM_001143850.3 99.8% 99.8% 266_267insGAA
3 human 79867 TCTN2 tectonic family member 2 XM_006719605.3 94.9% 94.9% 1986_1986delCins106
4 human 79867 TCTN2 tectonic family member 2 XM_005253623.2 93.5% 93.5% 1097_1098ins135
5 human 79867 TCTN2 tectonic family member 2 XM_017019974.1 93.4% 93.4% 266_267insGAA;1094_1095ins135
6 human 79867 TCTN2 tectonic family member 2 XM_017019975.1 56.3% 56.3% 0_1ins912
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2157
ORF length:
2091
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg cttccagcct ccggccgctc ttcttttgag gcttttcctt ctgcagggca
121tcctgaggct tctgtggggg gacctggctt tcatccctcc ttttatccga atgtccggcc
181ctgcggtcag cgcgtccctg gtcggagaca ccgagggtgt gaccgtgtcc ctggcagtgc
241tgcaggacga ggcgggaata ttgccaattc cgacgtgtgg agtgctgaac aatgagacgg
301aagactggag cgtgactgtg atccccggtg cgaaggtgtt ggaagtgaca gtgaggtgga
361agagaggtct ggactggtgt tcctccaatg agacagattc cttctcagag tccccctgta
421tcctccagac ccttctggtt tcagcatctc ataattcatc ctgttcagca catctactca
481ttcaagtgga aatttatgcc aactcttctc tgacccataa tgcctcagag aacgtgactg
541tcattcctaa ccaggtgtat cagccccttg gcccttgtcc ttgtaattta acagctggag
601cctgtgatgt tcgctgctgc tgtgaccagg aatgctcatc aaatttaaca acgctgttca
661gacggtcctg cttcaccggc gtgtttggag gagacgtcaa tcctcctttt gatcagctct
721gctctgctgg gacgacgaca cgtggtgtcc ccgattggtt tccctttctg tgtgtgcagt
781ccccccttgc caacacaccc ttccttggtt acttctatca tggtgctgtt tcccccaaac
841aggactcttc ctttgaagta tatgtggata ctgacgcaaa agactttgca gactttggtt
901acaaacaagg agatcccatt atgactgtaa agaaggcata ttttactatt ccgcaggtgt
961ccctggctgg gcagtgtatg cagaacgccc cagtggcatt tcttcacaat tttgatgtta
1021aatgcgttac taatttggaa ctataccaag aacgagatgg tattatcaat gcgaagataa
1081agaatgttgc cttaggaggc atagttacac caaaagtgat ctatgaggaa gcaactgacc
1141tagacaaatt catcaccaat acagaaactc ctttaaataa cggatcaacc cctagaattg
1201tgaatgtgga agaacattat attttcaaat ggaataataa taccatcagt gaaataaatg
1261ttaaaatttt tagggcagag attaatgccc accagaaagg gataatgaca cagagatttg
1321tagtaaaatt tttaagctat aatagtggta atgaagaaga attatctgga aatccaggtt
1381accaacttgg caagcctgtc cgagctctaa atatcaacag gatgaataat gtcacgactt
1441tacatctttg gcaatcggct ggaaggggtc tgtgtacatc agcaactttc aaacccattt
1501tatttggaga aaatgtactc tctggatgcc tgttagaagt cgggattaat gaaaattgta
1561ctcagctcag ggagaatgct gttgaaagac ttgattcatt aatacaagcg actcacgttg
1621caatgagagg caactccgat tacgctgatc ttagtgatgg ctggctcgaa ataatacgtg
1681tagatgcccc tgatccaggt gcagacccgc tggctagcag tgtgaacggc atgtgcctgg
1741atattcctgc tcacctgagc atccgcatcc tcatctcgga tgctggcgcg gtggaaggga
1801ttactcagca ggagatactc ggtgtagaga caaggttctc ctcagtgaac tggcagtacc
1861agtgtgggct tacctgtgag cacaaggccg accttctccc tatcagtgca tccgtccagt
1921ttattaaaat tcctgcacag ttaccccacc ccctgacaag attccagatc aattatacag
1981agtatgactg caacagaaat gaggtgtgtt ggccgcagct tctatatcca tggactcagt
2041attatcaagg ggagctgcat tctcagtgtg ttgctaaggg cttactgttg ctgttgttcc
2101tcacattggc cttgttcctc agcaacccct ggaccagaat atgcaaagcc tatagttacc
2161caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2221ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2281tta

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