Transcript: Human XM_005253623.2

PREDICTED: Homo sapiens tectonic family member 2 (TCTN2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TCTN2 (79867)
Length:
2779
CDS:
129..2087

Additional Resources:

NCBI RefSeq record:
XM_005253623.2
NBCI Gene record:
TCTN2 (79867)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005253623.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000129113 CTCCAATGAGACAGATTCCTT pLKO.1 446 CDS 100% 3.000 4.200 N TCTN2 n/a
2 TRCN0000147549 GCATCCGTCCAGTTTATTAAA pLKO.1 1836 CDS 100% 15.000 10.500 N TCTN2 n/a
3 TRCN0000418069 ATGAATAATGTCACGACTTTA pLKO_005 1350 CDS 100% 13.200 9.240 N TCTN2 n/a
4 TRCN0000148924 CAACTCTTCTCTGACCCATAA pLKO.1 563 CDS 100% 10.800 7.560 N TCTN2 n/a
5 TRCN0000129857 GAGAATGCTGTTGAAAGACTT pLKO.1 1500 CDS 100% 4.950 3.465 N TCTN2 n/a
6 TRCN0000128387 GAGATCCCATTATGACTGTAA pLKO.1 973 CDS 100% 4.950 3.465 N TCTN2 n/a
7 TRCN0000128166 CATCTACTCATTCAAGTGGAA pLKO.1 534 CDS 100% 2.640 1.848 N TCTN2 n/a
8 TRCN0000146621 CCTAGACAAATTCATCACCAA pLKO.1 1202 CDS 100% 2.640 1.848 N TCTN2 n/a
9 TRCN0000436903 GACCACAGATGCTCCACCATG pLKO_005 2511 3UTR 100% 1.350 0.810 N TCTN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005253623.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04140 pDONR223 100% 93.5% 93.5% None 1097_1098ins135 n/a
2 ccsbBroad304_04140 pLX_304 0% 93.5% 93.5% V5 1097_1098ins135 n/a
3 TRCN0000475922 TGTCTAGGGGTTATGGAACATGTC pLX_317 15.4% 93.5% 93.5% V5 1097_1098ins135 n/a
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