Construct: ORF ccsbBroad304_04335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018027.1_s304c1, BRDN0000402751
- Derived from:
- ccsbBroadEn_04335
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EVA1A (84141)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04335
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001135032.2 | 100% | 100% | |
| 2 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001369524.1 | 100% | 100% | |
| 3 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_001369525.1 | 100% | 100% | |
| 4 | human | 84141 | EVA1A | eva-1 homolog A, regulator ... | NM_032181.3 | 100% | 100% | |
| 5 | mouse | 232146 | Eva1a | eva-1 homolog A (C. elegans) | NM_145570.2 | 82.3% | 84.7% | (many diffs) |
| 6 | mouse | 232146 | Eva1a | eva-1 homolog A (C. elegans) | XM_006505962.1 | 82.3% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gctgcccctc agccacagcc cagagcacgt ggagatggct ttgctcagca 121 acatcctagc ggcctattcc tttgtctcag aaaatcctga gcgagcagct ctgtactttg 181 tttctggcgt gtgcatcggg ctggtgctga ccctggctgc tctggtgata aggatctctt 241 gccacacaga ctgcaggcgg cgtcccggga agaagttcct gcaggacaga gagagcagca 301 gcgacagcag cgacagcgag gatggcagtg aggacaccgt gtccgatctc tccgtgcgga 361 gacaccgccg cttcgagagg actttgaaca agaatgtgtt cacctctgcg gaggagctgg 421 agcgcgccca gcggctggag gagcgcgagc gcatcatcag ggagatctgg atgaatggcc 481 agcctgaggt gcccgggacc aggagcctga atcgctacta ttacccaact ttcttgtaca 541 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 601 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta