Construct: ORF ccsbBroad304_04589

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002833.1_s304c1, BRDN0000399065
Derived from:
ccsbBroadEn_04589
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DPH7 (92715)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04589
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92715 DPH7 diphthamide biosynthesis 7 NM_138778.5 100% 100%
2 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346370.2 95.1% 95.1% 707_708ins66
3 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346371.2 72.4% 69.9% (many diffs)
4 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346372.2 70.8% 69.6% (many diffs)
5 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346373.2 70.3% 65.1% 0_1ins310;63_64ins92
6 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346374.2 66% 64.8% (many diffs)
7 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346375.2 61% 61% 0_1ins528
8 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346376.2 61% 61% 0_1ins528
9 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346377.2 61% 61% 0_1ins528
10 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346378.2 61% 61% 0_1ins528
11 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346379.2 61% 61% 0_1ins528
12 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346380.2 61% 61% 0_1ins528
13 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346381.2 61% 61% 0_1ins528
14 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346382.2 61% 61% 0_1ins528
15 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346383.2 61% 61% 0_1ins528
16 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346384.2 61% 61% 0_1ins528
17 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346385.2 61% 61% 0_1ins528
18 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346386.2 61% 61% 0_1ins528
19 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346387.2 61% 61% 0_1ins528
20 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346388.2 61% 61% 0_1ins528
21 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346389.2 61% 61% 0_1ins528
22 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346390.2 61% 61% 0_1ins528
23 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346391.2 61% 61% 0_1ins528
24 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346392.2 61% 61% 0_1ins528
25 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346393.2 56.1% 56.1% 0_1ins528;179_180ins66
26 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346394.2 56.1% 56.1% 0_1ins528;179_180ins66
27 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346395.2 56.1% 56.1% 0_1ins528;179_180ins66
28 human 92715 DPH7 diphthamide biosynthesis 7 NM_001346396.2 56.1% 56.1% 0_1ins528;179_180ins66
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1422
ORF length:
1356
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat gggctgtttc gccctgcaaa cggtggacac cgagctgacc gcggactcgg
121tggagtggtg cccgctgcaa ggctgcaggc acctgctggc gtgcgggacc taccagctgc
181ggcggccgga ggaccggcct gccggccccc agaacaaggg tggaatggaa gttaaggagc
241ctcaggtccg tttaggccgt ctcttcctgt acagtttcaa tgacaacaac tctattcacc
301ctctggtcga ggtccaaaga aaagatactt ctgcaatcct ggacatgaaa tggtgtcaca
361tcccggtggc tggacatgcc ctcttgggct tggcagatgc cagtggatcc atacaactgc
421tccgcctggt ggaatctgag aagagccacg tgctggagcc attgtccagc cttgccctgg
481aggagcagtg tctggctttg tccctagatt ggtccactgg gaaaactgga agggccgggg
541accagccctt gaagatcatc agcagtgact ccacagggca gctccacctc ctgatggtga
601atgagacgag gcccaggctg cagaaagtgg cctcatggca ggcacatcaa ttcgaggcct
661ggattgctgc tttcaattac tggcatccag aaattgtgta ttcagggggc gacgatggcc
721ttctgagggg ctgggacacc agggtacccg gcaaatttct cttcaccagc aaaagacaca
781ccatgggtgt gtgcagcatc cagagcagcc ctcatcggga gcacatcctg gccacgggaa
841gctatgatga acacatccta ctgtgggaca cacgaaacat gaagcagccg ttggcagata
901cgcctgtgca gggtggggta tggagaatca agtggcaccc tttccaccac cacctgctcc
961tggccgcctg catgcacagt ggctttaaga tcctcaactg ccaaaaggca atggaggaga
1021ggcaggaggc gacggtcctg acatctcaca cattgcccga ctcgctggtg tatggagccg
1081actggtcctg gctgctcttc cgttctctgc agcgggcccc ctcgtggtcc tttcctagca
1141acctaggaac caagacggca gacctgaagg gtgcaagcga gttgccaaca ccctgtcatg
1201aatgcagaga ggataacgat ggggagggcc atgccagacc ccagagtgga atgaagccac
1261tcacagaggg catgaggaag aatggcacct ggctgcaggc tacagcagcc accacacgtg
1321actgtggcgt gaacccagaa gaagcagact cagccttcag cctcctggcc acctgctcct
1381tctatgacca tgcgctccac ctctgggagt gggaggggaa ctgcccaact ttcttgtaca
1441aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1501ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)