Construct: ORF ccsbBroad304_04614

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013134.1_s304c1, BRDN0000401691
Derived from:
ccsbBroadEn_04614
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SFXN5 (94097)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04614
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 94097 SFXN5 sideroflexin 5 NM_144579.3 100% 100%
2 human 94097 SFXN5 sideroflexin 5 NM_001371738.1 93.8% 84.8% 0_1ins61;107_108insGA
3 human 94097 SFXN5 sideroflexin 5 NM_001371739.1 93.2% 93.2% 100_101ins69
4 human 94097 SFXN5 sideroflexin 5 NM_001330400.2 92.2% 90.4% (many diffs)
5 human 94097 SFXN5 sideroflexin 5 NM_001371737.1 89.2% 83.6% 0_1ins61;109_163del
6 human 94097 SFXN5 sideroflexin 5 NM_001330406.2 84.4% 84% 0_1ins112;58_112del
7 human 94097 SFXN5 sideroflexin 5 NM_001371740.1 84.4% 72.1% 741_742ins86;861_862ins73
8 human 94097 SFXN5 sideroflexin 5 NM_001330402.2 82.6% 81.4% 827_828ins118;843_844ins59
9 human 94097 SFXN5 sideroflexin 5 NM_001371741.1 77% 71.1% 625_626ins116;711_712ins118
10 human 94097 SFXN5 sideroflexin 5 NM_001330401.2 74.4% 56% 625_626ins202;759_760ins59
11 human 94097 SFXN5 sideroflexin 5 NM_001330404.2 69.4% 69.4% 0_1ins312
12 human 94097 SFXN5 sideroflexin 5 NM_001330410.2 69.4% 69.4% 0_1ins312
13 human 94097 SFXN5 sideroflexin 5 NM_001330405.2 68% 66% (many diffs)
14 human 94097 SFXN5 sideroflexin 5 NM_001330407.2 63.3% 61.3% (many diffs)
15 human 94097 SFXN5 sideroflexin 5 NM_001371742.1 61.4% 61.1% 625_626insCCAGTGCCA;627_628ins384
16 human 94097 SFXN5 sideroflexin 5 NM_001330403.2 60.1% 42.7% (many diffs)
17 human 94097 SFXN5 sideroflexin 5 NM_001371743.1 59.7% 59.7% 534_535ins411
18 human 94097 SFXN5 sideroflexin 5 NM_001330411.2 55.5% 42.4% 0_1ins430;170_171ins23
19 human 94097 SFXN5 sideroflexin 5 NM_001330412.2 55.5% 42.4% 0_1ins430;170_171ins23
20 human 94097 SFXN5 sideroflexin 5 NM_001330408.2 52% 50.8% 0_1ins312;515_516ins118;531_532ins59
21 human 94097 SFXN5 sideroflexin 5 NR_138476.2 23.6% (many diffs)
22 mouse 94282 Sfxn5 sideroflexin 5 NM_178639.4 89.6% 97% (many diffs)
23 mouse 94282 Sfxn5 sideroflexin 5 XM_006506816.4 84.7% 91.4% (many diffs)
24 mouse 94282 Sfxn5 sideroflexin 5 XM_030255684.1 59.4% 64.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1089
ORF length:
1020
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggataca gcgactacag catcggcggc ggcggctagt gccgctagcg
121cctcgagcga tgcacctcct ttccaactgg gcaaaccccg cttccagcag acgtccttct
181atggccgctt caggcacttc ttggatatca tcgaccctcg cacactcttt gtcactgaga
241gacgtctcag agaggctgtg cagctgctgg aggactataa gcatgggacc ctgcgcccgg
301gggtcaccaa tgaacagctc tggagtgcac agaaaatcaa gcaggctatt ctacatccgg
361acaccaatga gaagatcttc atgccattta gaatgtcagg ttatattcct tttgggacgc
421caattgtagt cggtcttctc ttgcccaacc agacactggc atccactgtc ttctggcagt
481ggctgaacca gagccacaat gcctgtgtca actatgcaaa ccgcaatgcg accaagcctt
541cacctgcatc caagttcatc cagggatacc tgggagctgt catcagcgcc gtctccattg
601ctgtgggcct taatgtcctg gttcagaaag ccaacaagtt caccccagcc acccgccttc
661tcatccagag gtttgtgccg ttccctgctg tagccagtgc caatatctgc aatgtggtcc
721tgatgcggta cggggagctg gaggaaggga ttgatgtcct ggacagcgat ggcaacctcg
781tgggctcctc caagatcgca gcccgacacg ccctgctgga gacggcgctg acgcgagtgg
841tcctgcccat gcccatcctg gtgctacccc cgatcgtcat gtccatgctg gagaagacgg
901ctctcctgca ggcacgcccc cggctgctcc tccctgtgca aagcctcgtg tgcctggcag
961ccttcggcct ggccctgccg ctggccatca gcctcttccc gcaaatgtca gagattgaaa
1021catcccaatt agagccggag atagcccagg ccacgagcag ccggacagtg gtgtacaaca
1081aggggttgtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1141tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1201tcaacctctg gatta

Download FASTA (ORF) (Full)