Transcript: Human NM_001371742.1

Homo sapiens sideroflexin 5 (SFXN5), transcript variant 20, mRNA.

Source:
NCBI, updated 2019-07-23
Taxon:
Homo sapiens (human)
Gene:
SFXN5 (94097)
Length:
3696
CDS:
8..637

Additional Resources:

NCBI RefSeq record:
NM_001371742.1
NBCI Gene record:
SFXN5 (94097)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001371742.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412477 TCAAATCTGAGCCTTAGTTTC pLKO_005 1074 3UTR 100% 10.800 15.120 N SFXN5 n/a
2 TRCN0000421881 GCCAATTGTAGTCGGTCTTCT pLKO_005 358 CDS 100% 4.950 6.930 N SFXN5 n/a
3 TRCN0000060300 GTCAACTATGCAAACCGCAAT pLKO.1 446 CDS 100% 4.050 5.670 N SFXN5 n/a
4 TRCN0000413399 GTCAACTATGCAAACCGCAAT pLKO_005 446 CDS 100% 4.050 5.670 N Sfxn5 n/a
5 TRCN0000060298 GCCATTTAGAATGTCAGGTTA pLKO.1 322 CDS 100% 4.950 3.960 N SFXN5 n/a
6 TRCN0000060299 TCCCAATTAGAGCCGGAGATA pLKO.1 642 3UTR 100% 4.950 3.960 N SFXN5 n/a
7 TRCN0000060301 CTTCAGGCACTTCTTGGATAT pLKO.1 127 CDS 100% 10.800 7.560 N SFXN5 n/a
8 TRCN0000060302 CTTAATGTCCTGGTTCAGAAA pLKO.1 548 CDS 100% 4.950 3.465 N SFXN5 n/a
9 TRCN0000428457 GCCTTCACCTGCATCCAAGTT pLKO_005 475 CDS 100% 4.950 3.465 N SFXN5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001371742.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04614 pDONR223 100% 61.4% 61.1% None 625_626insCCAGTGCCA;627_628ins384 n/a
2 ccsbBroad304_04614 pLX_304 0% 61.4% 61.1% V5 625_626insCCAGTGCCA;627_628ins384 n/a
3 TRCN0000472753 CAGTTTCTAATAGCCCTAGATCTC pLX_317 34.5% 61.4% 61.1% V5 625_626insCCAGTGCCA;627_628ins384 n/a
4 ccsbBroadEn_09366 pDONR223 100% 61.3% 61.1% None 522C>T;625_626insCCAGTGCCA;627_628ins384 n/a
5 ccsbBroad304_09366 pLX_304 0% 61.3% 61.1% V5 522C>T;625_626insCCAGTGCCA;627_628ins384 n/a
6 TRCN0000475820 ATTCCTTCCGGTGTATCAGCCGTT pLX_317 14.5% 61.3% 61.1% V5 522C>T;625_626insCCAGTGCCA;627_628ins384 n/a
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