Construct: ORF ccsbBroad304_04644

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018237.1_s304c1, BRDN0000407620
Derived from:
ccsbBroadEn_04644
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF12 (113220)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04644
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 113220 KIF12 kinesin family member 12 NM_138424.1 100% 100%
2 human 113220 KIF12 kinesin family member 12 XM_024447404.1 100% 100%
3 human 113220 KIF12 kinesin family member 12 XM_006716947.2 80.7% 74.1% 1_312del;1715_1734del;1872_1905del
4 human 113220 KIF12 kinesin family member 12 XM_024447408.1 78.8% 78.8% 1_414del
5 human 113220 KIF12 kinesin family member 12 XM_024447406.1 78.4% 76.9% (many diffs)
6 human 113220 KIF12 kinesin family member 12 XM_005251683.5 76.6% 70.3% 1_414del;1817_1836del;1974_2007del
7 human 113220 KIF12 kinesin family member 12 XM_024447405.1 76.5% 73.3% (many diffs)
8 human 113220 KIF12 kinesin family member 12 XR_002956749.1 66.9% (many diffs)
9 human 113220 KIF12 kinesin family member 12 XR_002956751.1 66.2% 1_219del;1401_1566del;1660_1661ins264
10 human 113220 KIF12 kinesin family member 12 XR_002956750.1 64.1% (many diffs)
11 human 113220 KIF12 kinesin family member 12 XM_024447407.1 44.6% 24.6% 324_325ins157;651_731del;804_805ins659
12 mouse 16552 Kif12 kinesin family member 12 XM_017320000.1 39.7% 40.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1605
ORF length:
1539
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca gaggaccttc gcctggctgt tggaccgcgt gcagcacctg ggtgcccctg
121tcacccttcg cgcctcttat ctggagatct acaatgagca ggttcgggac ttgctgagcc
181tggggtctcc ccggcccctc cctgttcgct ggaacaagac tcggggcttc tatgtggagc
241agctgcgggt ggtggaattt gggagtctgg aggccctgat ggaacttttg caaacgggtc
301tcagccgtcg aaggaactca gcccacaccc tgaaccaggc ctccagccga agccatgccc
361tgctcaccct ttacatcagc cgtcaaactg cccagcagat gccttctgtg gaccctgggg
421agccccctgt tggtgggaag ctgtgctttg tggacctggc aggcagtgag aaggtagcag
481ccacgggatc ccgtggggag ctgatgcttg aggctaacag catcaaccga agcctgctgg
541ccctgggtca ctgcatctcc ctgctgctgg acccacagcg gaagcagagc cacatccctt
601tccgggacag caagctcacc aagttgctgg cagactcact gggagggcgc ggggtcaccc
661tcatggtggc ctgcgtgtcc ccctcagccc agtgccttcc tgagactctc agcaccctgc
721gatatgcaag ccgagctcag cgggtcacca cccgaccaca ggcccccaag tctcctgtgg
781caaagcagcc ccagcgtttg gagacagaga tgctgcagct ccaggaggag aaccgtcgcc
841tgcagttcca gctggaccaa atggactgca aggcctcagg gctcagtgga gcccgggtgg
901cctgggccca gcggaacctg tacgggatgc tacaggagtt catgctagag aatgagaggc
961tcaggaaaga aaagagccag ctgcagaata gccgagacct ggcccagaat gagcagcgca
1021tcctggccca gcaggtccat gcactagaga ggcgtctcct ctctgcctgc taccatcacc
1081agcagggtcc tggcctgacc ccaccgtgtc cctgcttgat ggccccagct cccccttgcc
1141atgcactgcc acccctctac tcctgcccct gctgccacat ctgcccactg tgtcgagtgc
1201ccctggccca ctgggcctgc ctgccagggg agcaccacct gccccaggtg ttggaccctg
1261aggcctcagg tggcaggccc ccatctgccc ggcccccacc ctgggcaccc ccatgcagcc
1321ctggctctgc caagtgccca agagagagga gtcacagtga ctggactcag acccgagtcc
1381tggcagagat gttgacggag gaggaggtgg taccttctgc acctcccctg cctgtgaggc
1441ccccgaagac atcaccaggg ctcagaggtg gggccggggt tccaaacctg gcccagagac
1501tggaggccct cagagaccag attggcagct ccctgcgacg tggccgcagc cagccaccct
1561gcagtgaggg cgcacggagc ccaggccaag tcctccctcc ccattgccca actttcttgt
1621acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1681gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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