Construct: ORF ccsbBroad304_04658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012207.1_s304c1, BRDN0000401559
- Derived from:
- ccsbBroadEn_04658
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERMAP (114625)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04658
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114625 | ERMAP | erythroblast membrane assoc... | NM_001017922.2 | 100% | 100% | |
| 2 | human | 114625 | ERMAP | erythroblast membrane assoc... | NM_018538.3 | 100% | 100% | |
| 3 | human | 114625 | ERMAP | erythroblast membrane assoc... | XM_006710313.4 | 100% | 100% | |
| 4 | human | 114625 | ERMAP | erythroblast membrane assoc... | XM_011540570.3 | 100% | 100% | |
| 5 | human | 114625 | ERMAP | erythroblast membrane assoc... | XM_011540571.3 | 98.5% | 98.5% | 636_637ins21 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1494
- ORF length:
- 1425
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatggcg agttctgctg gctcctggct ctctggctgc ctcatccctc 121 tcgtcttcct ccggctgtct gtgcatgtgt caggccacgc aggggatgcc ggcaagttcc 181 acgtggccct actagggggc acagccgagc tgctctgccc tctctccctc tggcccggga 241 cggtacccaa ggaggtgagg tggctgcggt ccccattccc gcagcgctcc caggctgttc 301 acatattccg ggatgggaag gaccaggatg aagatctgat gccggaatat aaggggagga 361 cggtgctagt gagagatgcc caagagggaa gtgtcactct gcagatcctt gacgtgcgcc 421 ttgaggacca agggtcttac cgatgtctga tccaagttgg aaatctgagt aaagaggaca 481 ccgtgatcct gcaggttgca gccccatctg tggggagtct ctccccctca gcagtggctc 541 tggctgtgat cctgcctgtc ctggtacttc tcatcatggt gtgcctttgc cttatctgga 601 agcaaagaag agcaaaagaa aagcttctct atgaacatgt gacggaggtg gacaatcttc 661 tttcagacca tgctaaagaa aaaggaaaac tccataaagc tgtcaagaaa ctccggagtg 721 aactgaagtt gaaaagagct gcagcaaact caggctggag aagagcccgg ttgcattttg 781 tggcagtgac cctggaccca gacacagcac atcccaaact catcctttct gaggaccaaa 841 gatgtgtaag gcttggagac agacggcagc ctgtacctga caacccccag agatttgatt 901 tcgttgtcag catcctaggc tctgagtact tcacgactgg ctgccactac tgggaggtgt 961 atgtgggaga caagaccaaa tggattcttg gagtatgtag tgagtcagtg agcaggaagg 1021 ggaaggttac tgcctcacct gccaatggac actggcttct gcgacagagt cgtgggaatg 1081 agtatgaagc tctcacatcc ccgcagacct ccttccgcct taaagagcct ccacggtgtg 1141 tggggatttt cctggactat gaagcaggag tcatctcttt ctacaatgtg accaacaagt 1201 cccacatctt tactttcacc cacaatttct ctggccccct tcgccctttc tttgaacctt 1261 gccttcatga tggaggaaaa aacacagcac ctctagtcat ttgttcagaa ctacacaaat 1321 cagaggaatc aattgtcccc aggccagaag ggaaaggcca tgctaatgga gatgtgtccc 1381 tcaaggtgaa ctcttcttta ctacccccga aggccccaga gctgaaggat ataatcctgt 1441 ccttgccccc tgaccttggc ccagcccttc aggagctcaa ggctccttct tttttgccaa 1501 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1561 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta