Construct: ORF ccsbBroad304_04687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004603.1_s304c1, BRDN0000399660
- Derived from:
- ccsbBroadEn_04687
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IQCD (115811)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04687
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115811 | IQCD | IQ motif containing D | NM_138451.3 | 100% | 100% | |
| 2 | human | 115811 | IQCD | IQ motif containing D | XM_011537864.2 | 93.5% | 93.5% | 728_799del |
| 3 | human | 115811 | IQCD | IQ motif containing D | NM_001330452.2 | 77.2% | 77.2% | 728_1033del |
| 4 | human | 115811 | IQCD | IQ motif containing D | XM_005253833.4 | 77.2% | 77.2% | 728_1033del |
| 5 | human | 115811 | IQCD | IQ motif containing D | XM_011537862.2 | 77.2% | 77.2% | 728_1033del |
| 6 | human | 115811 | IQCD | IQ motif containing D | XM_011537863.2 | 77.2% | 77.2% | 728_1033del |
| 7 | human | 115811 | IQCD | IQ motif containing D | XM_005253834.4 | 64.7% | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1107
- ORF length:
- 1041
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tttagacatt ctcgccatgg cccctttgta tcaggcccct gccatcaaca 121 gaatagggcc aaagacagac ccatctaaaa ggccagctga tccactaaaa cccttggttc 181 tctctaggac caaactcacc accattgagg ccaaaaggat catgtccatc ctggatgagg 241 cgatctacaa ggtggagctg gtgaccttgc tgtcgtatgt ggcatccaac agagaggata 301 tggaggggat gctgggggag gacgtcatga gggcagtgag agagcatgag gatctctgcc 361 aggtcctcct tgaaaacgtc aggtgcctga aagagaaaga aaggcaattg caggagcaga 421 aagaggctga ggaggaaggg tggctcagag accgcctcct ttccatagag ctgcagaaat 481 ccagcctctc accacttatg cagcagatca aagactccac caagaacgtc cttagactct 541 tgctcagcaa cccccaggct gctaggctcc tgcagatgca gacacagggt agaagtgcag 601 aagcccagaa ttttattgac agcctgatag agctccgtgg tttcctgttt gaaaaactac 661 tcactagtcc catggaagcg agggataagg cccagttctt acaggatatc agtagacaga 721 atagtaataa ccaacaaatc attgatactc ttgaaaagga attggcggag agaatgaaga 781 acaggaatgc agaggaggag ttggaggatc tggacgctgt tcacagggag gagaagatct 841 cgctggagga gctcaggcgg aggcacaaag tgctggtggg agagtttgcg cagatccggg 901 aagagcggga gatcaactcc aagaaaagga tggaggcaga gcaggagatg gtgcgcatgg 961 tacgggcggc cacgctcatc caggccctat ggaagggcta tctggtgcgc tccctgctca 1021 gatccaagaa gaagcggggc aagggcaaag caaaggacaa ggagaagggc aagcagaaag 1081 gcaaggagaa gggcaagggc aagaaatgcc caactttctt gtacaaagtg gttggtaagc 1141 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1201 ggcgcgttaa gtcgacaatc aacctctgga tta