Construct: ORF ccsbBroad304_04728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011818.1_s304c1, BRDN0000411036
- Derived from:
- ccsbBroadEn_04728
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TWIST2 (117581)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04728
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 117581 | TWIST2 | twist family bHLH transcrip... | NM_001271893.4 | 100% | 100% | |
| 2 | human | 117581 | TWIST2 | twist family bHLH transcrip... | NM_057179.3 | 100% | 100% | |
| 3 | mouse | 13345 | Twist2 | twist basic helix-loop-heli... | NM_007855.3 | 96.6% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagggctcc agctcgcccg tgtcccccgt ggacagcctg ggcaccagcg 121 aggaggagct cgagaggcag cccaagcgct tcggccggaa gcggcgctac agcaagaagt 181 cgagcgaaga tggcagcccg accccgggca agcgcggcaa gaagggcagc cccagcgcgc 241 agtccttcga ggagctgcag agccagcgca tcctggccaa cgtgcgcgag cgccagcgca 301 cccagtcgct caacgaggcc ttcgcggcgc tgcgcaagat catccccacg ctgccctctg 361 acaagctgag caagatccag acgctcaagc tggccgccag gtacatagac ttcctctacc 421 aggtcctgca gagcgacgag atggacaata agatgaccag ctgcagctac gtggcccacg 481 agcgcctcag ctacgccttc tccgtgtggc gcatggaggg cgcgtggtcc atgtccgcct 541 cccactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 601 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 661 acctctggat ta