Construct: ORF ccsbBroad304_04838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002582.1_s304c1, BRDN0000410869
- Derived from:
- ccsbBroadEn_04838
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL55 (128308)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04838
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_001321284.1 | 100% | 100% | |
| 2 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181441.2 | 100% | 100% | |
| 3 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181454.2 | 100% | 100% | |
| 4 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181455.2 | 100% | 100% | |
| 5 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181456.2 | 100% | 100% | |
| 6 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181463.3 | 100% | 100% | |
| 7 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181464.2 | 100% | 100% | |
| 8 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181465.2 | 100% | 100% | |
| 9 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_005273059.4 | 100% | 100% | |
| 10 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_005273061.5 | 100% | 100% | |
| 11 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_005273062.3 | 100% | 100% | |
| 12 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_005273063.5 | 100% | 100% | |
| 13 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_011544095.3 | 100% | 100% | |
| 14 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XM_024453282.1 | 100% | 100% | |
| 15 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | NM_181462.2 | 78% | 78% | 26_133del |
| 16 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XR_002959481.1 | 11.7% | 1_2652del;2678_2838del;3198_3273del | |
| 17 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XR_002959482.1 | 11.1% | (many diffs) | |
| 18 | human | 128308 | MRPL55 | mitochondrial ribosomal pro... | XR_002959480.1 | 11.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccgtgggc agcctgcttg gccggctgag gcagagcacc gtgaaggcca 121 ccggacctgc actccgccgc ctgcacacat cctcctggcg agctgacagc agcagggcct 181 cactcactcg tgtgcaccgc caggcttatg cacgactcta ccccgtgctg ctggtgaagc 241 aggatggctc caccatccac atccgctaca gggagccacg gcgcatgctg gcgatgccca 301 tagatctgga caccctgtct cctgaggagc gccgggccag gctgcggaag cgtgaggctc 361 agctccagtc gaggaaggag tacgagcagg agctcagtga tgacttgcat gtggagcgct 421 accgacagtt ctggaccagg accaagaagt gcccaacttt cttgtacaaa gtggttggta 481 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 541 ggtggcgcgt taagtcgaca atcaacctct ggatta