Construct: ORF ccsbBroad304_04849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015663.1_s304c1, BRDN0000401378
- Derived from:
- ccsbBroadEn_04849
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR1-1HG (128826)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04849
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128826 | MIR1-1HG | MIR1-1 host gene | NM_001302812.2 | 100% | 100% | |
| 2 | human | 128826 | MIR1-1HG | MIR1-1 host gene | NM_178463.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccagctgt agctgtgcat tgatggcgcc gtgcggcccg gccgcaggtc 121 ccgcagccgt ggagaggacc cagcaggtgg cgcggggaga gcccggctcg gcacgtggtc 181 agctccaagt cagccctgaa atgtccatta ctcacaaaga aaaggaaaat gctcatttga 241 aagaaattct tctctttgtc aatgctgagg cattctctca gccccagccc cacagtgctc 301 cagtgtgtga agggcaacag ctgacgggga agttcagcac atctgtgctg accagggctg 361 gaggcgatgc cagtccgtgc agctgggaac gcctcctctg ctatggctgg agccactgct 421 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta