Construct: ORF ccsbBroad304_04859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000573.1_s304c1, BRDN0000411857
- Derived from:
- ccsbBroadEn_04859
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFTN2 (130132)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04859
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130132 | RFTN2 | raftlin family member 2 | NM_144629.3 | 100% | 100% | |
| 2 | human | 130132 | RFTN2 | raftlin family member 2 | XM_011510597.3 | 99.8% | 99.8% | 1233_1234insAAG |
| 3 | human | 130132 | RFTN2 | raftlin family member 2 | XM_011510596.3 | 98.2% | 98.2% | 1232_1258del |
| 4 | human | 130132 | RFTN2 | raftlin family member 2 | XM_011510595.3 | 98% | 98% | 1234_1263del |
| 5 | human | 130132 | RFTN2 | raftlin family member 2 | XM_017003332.2 | 96.6% | 96.6% | 436_437ins51 |
| 6 | human | 130132 | RFTN2 | raftlin family member 2 | XM_011510598.3 | 94.7% | 94.7% | 436_437ins51;1183_1212del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1572
- ORF length:
- 1503
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtgcgga cttagaaagc tagaagaccc tgatgatagc agccctggaa 121 aaatattttc taccctgaag agaccgcaag tggaaacaaa gacagaattt gcttacgaat 181 atgtattgct ggattttact ctacaagctt catcaaaccc agaagtcatc aagataaatt 241 caattctgga tatagtaaca aaagtggaaa actattatct taaaggatat attgtcgggg 301 ctattcatcc tgttatacaa cctgtggggc agcgaaaaca cctacctgca agttacctat 361 acagagtggt gctattgcgc ttgaaattaa gcccaaagaa ttcggcagca cccagtggac 421 aaagacgccc aaggctcgtg attgaggaat gtcccctaac ttctgaggca caaacaaatg 481 acgcagcaaa agaactgata gaaaagatta atgtcgctgc taaaagagga atgaaatttg 541 ttggattcat atcacagcat tattctccat ctaaattctg caatggaacc aaccatgatg 601 gagatataga atcgatgcta catgtgagac acggttcaga tgaaaactgt agaagttgga 661 atgaagggac gttaagtggg cagtcatctg aaagcggaat tgaggaagaa cttcatcatg 721 aaagtggaca gtatcaaatg gaacaaaatg gcagccctac ttcctctaaa tcaagaaagg 781 gagaagcctc agataacaaa ttgtatacag tcttcaatgc ttttgatgat gattcaacct 841 cctgggccta tcaggaaggc atcctgtcaa tgaaagtaac aagaaaagga tcagtcatta 901 gtacacttga tgctgattgg ctggagttaa ctacctttta ttataaacaa ggcttgtctt 961 taattgattc ttttgtattc tgggaaacat ctaaagggga acatttgcct aaatctttgg 1021 aaggattttt tatctatgaa gaagaaggtt ctggagttcc aggttctagt aggaaaggaa 1081 atgatgccat cgtagtagaa caatggactg ttattgaggg atgtgaaatc aaaacagatt 1141 atggccctct gctgcacact ctggctgaat tcggatggct tctgacaagc gtgttgccca 1201 cacctgtatt gagacatgac agtgaaggga atttggctac aaagcagatc gtattccttc 1261 agaggccagt tatgtggaat tcagctgctc aaacaccaga taagaaagcc agccgccaca 1321 taaaaggtga agacaagaat aaagccacta gtagaagcat cggattagac acaacctcgt 1381 cacaacctgc agagagcaga cacctgcctg aggagtgccg cctttctccc tcccgggaat 1441 gctggacaaa ggagggaagg ctggcacagc acaacagctt ctctgggttc agcagcagtg 1501 acaacgtcct ccgggaatta gacgacggac agtttgatca ggaagatgga gtgactcagg 1561 tcacttgtat gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1621 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1681 caatcaacct ctggatta