Construct: ORF ccsbBroad304_04915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003870.1_s304c1, BRDN0000402576
- Derived from:
- ccsbBroadEn_04915
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf116 (138162)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04915
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138162 | C9orf116 | chromosome 9 open reading f... | NM_144654.3 | 100% | 100% | |
| 2 | human | 138162 | C9orf116 | chromosome 9 open reading f... | NM_001048265.2 | 67.6% | 55.7% | 223_268del;323_408del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 342
- ORF length:
- 276
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaggaatgc cccagagcgt gcgcggagcc tgtggcgccc aaggccacgg 121 ccccgccgga gaggaccagc gactactacc gcgtgagcgc ggacctgccg ggcaggttca 181 acaacccggg gtggttccgg ggctacagga cccagaaggc tgtctccgtg tacaggacca 241 gtaaccaggc ttacgggagc agagccccca ccgtgcacga gatgcctcgg gtggaatgtt 301 ccggaacaat actctcaatg tttacctgga gaaaagcatc gtgcccaact ttcttgtaca 361 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 421 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta