Construct: ORF ccsbBroad304_04925

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013462.1_s304c1, BRDN0000411711
Derived from:
ccsbBroadEn_04925
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PWWP3B (139221)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04925
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 139221 PWWP3B PWWP domain containing 3B NM_001171020.2 100% 100%
2 human 139221 PWWP3B PWWP domain containing 3B NM_152423.4 100% 100%
3 human 139221 PWWP3B PWWP domain containing 3B XM_005262077.2 100% 100%
4 human 139221 PWWP3B PWWP domain containing 3B XM_011530856.1 100% 100%
5 human 139221 PWWP3B PWWP domain containing 3B XM_011530857.1 100% 100%
6 human 139221 PWWP3B PWWP domain containing 3B XM_017029269.1 100% 100%
7 human 139221 PWWP3B PWWP domain containing 3B XM_024452334.1 100% 100%
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2157
ORF length:
2088
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggagtctgag tatgtcctat gcaactggaa agaccagttg tggccagcaa
121aagttttgtc cagatctgaa acttcatcaa acagtaagag gaaaaaggca ttttctctag
181aagttcaaat actctcacta gatgaaaaaa ttaaattgga cagcacagaa acaaagatcc
241taaataaatc tcaaattgaa gccattgctg cctcattagg actacagtca gaggacagtg
301ctccacctac agaggaaact gcctatggaa gatcactaaa agtggcactg ggtattctga
361atgagagaac aaatttgagt caagcaagca cttcagatga agaggagatc actatgctgt
421ctcaaaatgt accacaaaaa cagtccgatt caccccctca taaaaaatac cggaaggatg
481aaggtgactt accagggtgt cttgaggaaa gggaaaactc agcatgcttg ttagcatctt
541cagagagtga tgattccctg tatgatgata aatcacaagc acccacaatg gtcgatacta
601ttccaagtga agtggaaaca aagtcattac aaaactctag ctggtgcgag actttccctt
661cactttcgga agataatgat gaaaaagaga acaagaataa gattgatatc tcagcagtta
721tgtctgtgca ttctgcagtc aaagaggaaa gtgcatgtgt taaagatgaa aagtttgctc
781cacctttgtc acctttgtca tcagatatgc tcattatgcc caaagctttg aaagaagaga
841gcgaggatac ctgcctagag accctggctg ttccctctga atgctctgct ttctcagaga
901atattgagga tcctggagag ggtccctcaa atccatgctt agataccagc cagaatcaac
961cttccatgga atcagagatg ggggctgcag catgccctgg gagttgttca agggaatgcg
1021aggtttcatt tagtgcctct aaccctgtct gggattattc acatcttatg agtagtgaaa
1081gaaattttca gagactggat tttgaagaac ttgaggaaga aggtcaagcc tctgacaagt
1141cattgcttcc aagtcgcatt aatctttctc tattagatga tgatgaggaa gacgaagaac
1201ttccacgctt cattttacat tatgagacac atccgtttga aacaggaatg atagtctggt
1261ttaaatatca gaaatatcca ttttggccag cagtgataaa aagtatcaga cgaaaagaga
1321ggaaagcaag tgtgcttttt gttgaggcaa acatgaattc tgaaaagaag ggcattagag
1381taaattttag aagattaaag aaatttgatt gtaaagagaa acaaatgcta gtggacaaag
1441ccagggagga ttatagtgag agtattgact ggtgcatctc actaatttgt gactacagag
1501ttagaatagg ttgtggttct ttcacgggct ctttgcttga gtattatgct gctgatatta
1561gttacccagt taggaaagaa acaaaacagg atactttcag gaacaaattt ccaaagctgc
1621ataatgaaga tgccagggaa ccgatggctg taacttccca gaccaagaaa atgtccttcc
1681aaaaaattct ccctgaccgg atgaaggctg ctcgggaccg agccaacaag aacctggtgg
1741acttcattgt gaatgcaaag ggaacagaga accatcttct ggccattgta aatggcacaa
1801aaggatccag atggctgaaa tcatttttga atgcaaatag gttcacaccc tgtattgaaa
1861catactttga ggatgaagat cagttggatg aagtggtgaa atatttacaa gaagtctgca
1921atcaaataga tcaaataatg ccaacttgga taaaagatga taaaattaaa tttatcctag
1981aagttcttct gccagaagca attatttgtt caatttctgc tgttgatggg ttagattacg
2041aggcagctga agcaaagtat ctaaaaggac catgtctagg ctacagggaa agagaattat
2101ttgatgcaaa aataatatat gaaaagagac gaaaagcacc aacaaatgaa gctcacttgc
2161caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2221ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2281tta

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