Construct: ORF ccsbBroad304_04943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001064.1_s304c1, BRDN0000406556
- Derived from:
- ccsbBroadEn_04943
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM69 (140691)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_04943
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140691 | TRIM69 | tripartite motif containing 69 | NM_080745.5 | 100% | 100% | |
| 2 | human | 140691 | TRIM69 | tripartite motif containing 69 | NM_001301144.2 | 86.8% | 86.8% | 0_1ins135 |
| 3 | human | 140691 | TRIM69 | tripartite motif containing 69 | NM_001301145.2 | 81.8% | 81.8% | 0_1ins135;150_151ins51 |
| 4 | human | 140691 | TRIM69 | tripartite motif containing 69 | NM_001301146.2 | 77.1% | 77.1% | 102_103ins234 |
| 5 | human | 140691 | TRIM69 | tripartite motif containing 69 | NM_182985.5 | 68% | 67.8% | 1_463del;467_482delinsGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggagctt gccatccaac agggtcaact ggagacaact ctgaaggagc 121 ttcagaccct gaggaacatg cagaaggaag ctattgctgc tcacaaggaa aacaagctac 181 atctgcagca acatgtgtcc atggagtttc taaagctgca tcagttcctg cacagcaaag 241 aaaaggacat tttaactgag ctccgggaag aggggaaagc cttgaatgag gagatggagt 301 tgaatctgag ccagcttcag gagcaatgtc tcttagccaa ggatatgttg gtgagcattc 361 aggcaaagac ggaacaacag aactccttcg actttctcaa agacatcaca actctcttac 421 atagcttgga gcaaggaatg aaggtgctgg caaccagaga gcttatttcc agaaagctga 481 acctgggcca gtacaaaggt cctatccagt acatggtatg gagggaaatg caggacactc 541 tctgcccagg cctgtctcca ctaactctgg accctaaaac agctcaccca aatctggtgc 601 tctccaaaag ccaaaccagc gtctggcatg gtgacattaa gaagataatg cctgatgatc 661 ctgagaggtt tgactcaagt gtggctgtac tgggctcaag aggcttcacc tctggaaagt 721 ggtactggga agtagaagta gcaaagaaga caaaatggac agttggagtt gtcagagaat 781 ccatcattcg gaagggcagc tgtcctctaa ctcctgagca aggattctgg cttttaagac 841 taaggaacca aactgatcta aaggctctgg atttgccttc tttcagtctg acactgacta 901 acaacctcga caaggtgggc atatacctgg attatgaagg aggacagttg tccttctaca 961 atgctaaaac catgactcac atttacacct tcagtaacac tttcatggag aaactttatc 1021 cctacttctg cccctgcctt aatgatggtg gagagaataa agaaccattg cacatcttac 1081 atccacagta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1141 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1201 tcaacctctg gatta