Construct: ORF ccsbBroad304_04957

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014454.1_s304c1, BRDN0000411876
Derived from:
ccsbBroadEn_04957
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SREK1 (140890)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_04957
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 140890 SREK1 splicing regulatory glutami... NM_001323527.2 100% 100%
2 human 140890 SREK1 splicing regulatory glutami... NM_139168.3 100% 100%
3 human 140890 SREK1 splicing regulatory glutami... XM_011543171.3 100% 100%
4 human 140890 SREK1 splicing regulatory glutami... NM_001270492.2 99.8% 99.8% 1230_1231insAGC
5 human 140890 SREK1 splicing regulatory glutami... NM_001077199.3 81.4% 81.4% 1_348del
6 human 140890 SREK1 splicing regulatory glutami... NM_001323529.2 81.2% 81.2% 1_348del;1578_1579insAGC
7 human 140890 SREK1 splicing regulatory glutami... NM_001323534.2 70.6% 70.6% 0_1ins447
8 human 140890 SREK1 splicing regulatory glutami... XM_017009056.1 70.6% 70.6% 0_1ins447
9 human 140890 SREK1 splicing regulatory glutami... NM_001323535.2 70.4% 70.4% 0_1ins447;783_784insAGC
10 human 140890 SREK1 splicing regulatory glutami... NM_001323533.2 67.6% 66% 1_348del;1580_1581ins145;1614_1615ins113
11 human 140890 SREK1 splicing regulatory glutami... XM_017009055.2 62% 62% 1_348del;1120_1121ins363
12 human 140890 SREK1 splicing regulatory glutami... XR_948236.3 56.4% 1_422del;957_1131del;1794_1795ins327
13 mouse 218543 Srek1 splicing regulatory glutami... NM_172592.2 85% 86.3% (many diffs)
14 mouse 218543 Srek1 splicing regulatory glutami... XM_006517607.3 85% 86.3% (many diffs)
15 mouse 218543 Srek1 splicing regulatory glutami... XM_017315497.1 85% 86.3% (many diffs)
16 mouse 218543 Srek1 splicing regulatory glutami... XM_006517606.2 69.3% 70.3% (many diffs)
17 mouse 218543 Srek1 splicing regulatory glutami... XM_006517608.3 58.2% 58.5% (many diffs)
18 mouse 218543 Srek1 splicing regulatory glutami... XM_017315498.1 58.2% 58.5% (many diffs)
19 mouse 218543 Srek1 splicing regulatory glutami... XR_001780780.1 31.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1593
ORF length:
1524
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacaagtctg atgcctggtg caggattgct tccaataccg accccaaatc
121ctttgactac tcttggtgtt tcacttagca gtttgggagc tataccagca gcagcactag
181accccaacat tgcaacactt ggagagatac cacagccacc acttatggga aacgtggatc
241cttccaaaat agatgaaatt aggagaacgg tttatgttgg aaatctgaat tcccagacaa
301cgacagctga tcaactactt gaatttttta aacaagttgg agaagtgaag tttgtgcgga
361tggcaggtga tgagactcag ccaactcggt ttgcttttgt ggaatttgca gaccaaaatt
421ctgtaccaag ggcccttgct tttaatggag ttatgtttgg agacaggcca ctgaaaataa
481atcactccaa caatgcaata gtaaaacccc ctgagatgac acctcaggct gcagctaagg
541agttagaaga agtaatgaag cgagtacgag aagctcagtc atttatctca gcagctattg
601aaccagagtc tggaaagagc aatgaaagaa aaggcggtcg atctcgttcc catactcgct
661caaaatccag gtctagctca aaatcccatt ctagaaggaa aagatcacaa tcaaaacaca
721ggagtagatc ccataataga tcacgttcaa gacagaaaga cagacgtaga tctaagagcc
781cacataaaaa acgctctaaa tcaagggaga gacggaagtc aaggagtcgt tcgcattcac
841gggacaagag aaaagacact cgagaaaaga tcaaggaaaa ggaaagagtg aaagagaaag
901acagggaaaa ggagagagag agggaaaagg aacgtgaaaa agaaaaggaa cggggtaaaa
961acaaagaccg ggacaaggaa cgggaaaagg accgggaaaa agacaaggaa aaggacagag
1021agagagaacg ggaaaaagag catgagaagg atcgagacaa agagaaggaa aaggaacagg
1081acaaagaaaa ggaacgagaa aaagacagat ccaaagagat agatgaaaaa agaaagaagg
1141ataaaaaatc cagaacacca cccaggagtt acaatgcatc gcgaagatct cgtagttcca
1201gcagggaaag gcgtaggagg aggagcagga gttcttccag atcgccaaga acatcaaaaa
1261ccataaaaag gaaatcttct agatctccgt cccccaggag cagaaataag aaggataaaa
1321agagagaaaa agaaagggac cacatcagtg aaagaagaga gagagaacgt tcaacgtcta
1381tgagaaagag ttctaatgat agagatggga aggagaagtt ggagaagaac agtacttcac
1441ttaaagagaa agagcacaat aaagaaccag attcaagtgt gagcaaagaa gtagatgaca
1501aggatgcacc aaggactgag gaaaacaaaa tacagcacaa tgggaattgt cagctgaatg
1561aagaaaacct ctctaccaaa acagaagcag tattgccaac tttcttgtac aaagtggttg
1621gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1681accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)