Transcript: Mouse XR_001780780.1

PREDICTED: Mus musculus splicing regulatory glutamine/lysine-rich protein 1 (Srek1), transcript variant X7, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Srek1 (218543)
Length:
4115
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001780780.1
NBCI Gene record:
Srek1 (218543)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001780780.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000123743 ACAGGAGTAGATCCCATAATA pLKO.1 1337 3UTR 100% 15.000 21.000 N Srek1 n/a
2 TRCN0000314570 ACAGGAGTAGATCCCATAATA pLKO_005 1337 3UTR 100% 15.000 21.000 N SREK1 n/a
3 TRCN0000123742 GCTGATCAACTACTTGAATTT pLKO.1 737 3UTR 100% 13.200 18.480 N Srek1 n/a
4 TRCN0000298293 GCTGATCAACTACTTGAATTT pLKO_005 737 3UTR 100% 13.200 18.480 N Srek1 n/a
5 TRCN0000294640 GATTCCACAGCCGCCACTTAT pLKO_005 637 3UTR 100% 13.200 10.560 N Srek1 n/a
6 TRCN0000294637 GCAGCGCTGGACCCAAATATT pLKO_005 602 3UTR 100% 15.000 10.500 N Srek1 n/a
7 TRCN0000294701 TTGAGAGAGAAATCCATATAT pLKO_005 2232 3UTR 100% 15.000 10.500 N Srek1 n/a
8 TRCN0000001311 CTCCAACAATGCAATAGTAAA pLKO.1 916 3UTR 100% 13.200 9.240 N SREK1 n/a
9 TRCN0000314568 CTCCAACAATGCAATAGTAAA pLKO_005 916 3UTR 100% 13.200 9.240 N SREK1 n/a
10 TRCN0000123741 GCAGCTAAGGAGTTAGAAGAA pLKO.1 962 3UTR 100% 4.950 3.465 N Srek1 n/a
11 TRCN0000123739 GCCTTCTACAATGTGTGGTAT pLKO.1 3001 3UTR 100% 4.950 3.465 N Srek1 n/a
12 TRCN0000123740 CGAAGGTAAAGTACAGCACAA pLKO.1 2088 3UTR 100% 4.050 2.835 N Srek1 n/a
13 TRCN0000287194 CGAAGGTAAAGTACAGCACAA pLKO_005 2088 3UTR 100% 4.050 2.835 N Srek1 n/a
14 TRCN0000001308 GAAGAAGTAATGAAGCGAGTA pLKO.1 977 3UTR 100% 4.050 2.835 N SREK1 n/a
15 TRCN0000314569 GAAGAAGTAATGAAGCGAGTA pLKO_005 977 3UTR 100% 4.050 2.835 N SREK1 n/a
16 TRCN0000350302 TTAGCAGTTTGGGAGCTATAC pLKO_005 576 3UTR 100% 10.800 7.560 N SREK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001780780.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04957 pDONR223 100% 31.3% None (many diffs) n/a
2 ccsbBroad304_04957 pLX_304 0% 31.3% V5 (many diffs) n/a
3 TRCN0000465487 AGTTACTTACTTAGACCATTCTAT pLX_317 17% 31.3% V5 (many diffs) n/a
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