Construct: ORF ccsbBroad304_05033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004898.1_s304c1, BRDN0000401066
- Derived from:
- ccsbBroadEn_05033
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHLD1 (149840)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05033
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149840 | SHLD1 | shieldin complex subunit 1 | NM_001303477.1 | 100% | 100% | |
| 2 | human | 149840 | SHLD1 | shieldin complex subunit 1 | NM_152504.4 | 100% | 100% | |
| 3 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_017027684.1 | 100% | 100% | |
| 4 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_006723549.4 | 99% | 99% | 1_6delATGACT |
| 5 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529177.2 | 94% | 94% | 1_39del |
| 6 | human | 149840 | SHLD1 | shieldin complex subunit 1 | NM_001303478.1 | 77.9% | 73.6% | (many diffs) |
| 7 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529178.2 | 35.1% | 28.1% | (many diffs) |
| 8 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529181.3 | 34.4% | 31.2% | (many diffs) |
| 9 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529179.2 | 32.3% | 29.3% | (many diffs) |
| 10 | human | 149840 | SHLD1 | shieldin complex subunit 1 | NM_001303479.2 | 29.9% | 29.2% | (many diffs) |
| 11 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529180.2 | 28.1% | 27.5% | (many diffs) |
| 12 | human | 149840 | SHLD1 | shieldin complex subunit 1 | XM_011529182.2 | 27.6% | 27.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc agccagggac gccacttcag gcagcctgtc agaggagagc agtgctttgg 121 acctgccatc agcgtgtgac ataagagatt acgtcctgca gggacccagc caagaagcca 181 acagcgaggc tttcagttct ttggaattcc attcttttcc ttattcttct gatgtggatc 241 cagacaccag taacttaaat atagaacaaa ataactcctg gaccgctgag aacttctggc 301 ttgaccctgc tgtgaaaggc cagtcagaga aggaagagga tgatggcctt cggaaatccc 361 tggatagatt ctatgaaatg tttggtcatc cacagccagg ctctgcaaac tcactctctg 421 catctgtctg caagtgcctg tctcagaaaa tcactcaact aagaggccag gagagccaaa 481 agtatgccct ccgcagtttt caaatggccc gggtgatctt caaccgggac ggctgctccg 541 tcttacagag gcattccagg gacacccact tctacccact ggaggaagga agtacatctt 601 tggatgatga aaagccaaac ccaggactgt caaaggatat tactcatttc ctcttgcagc 661 agaatgtaat gaaagacctg tacccaactt tcttgtacaa agtggttggt aagcctatcc 721 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 781 ttaagtcgac aatcaacctc tggatta