Construct: ORF ccsbBroad304_05042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008699.1_s304c1, BRDN0000411445
- Derived from:
- ccsbBroadEn_05042
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDPF1 (150383)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05042
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | NM_207327.5 | 100% | 100% | |
| 2 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529965.2 | 100% | 100% | |
| 3 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529963.2 | 70.2% | 38.4% | (many diffs) |
| 4 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529964.2 | 67.4% | 33.9% | (many diffs) |
| 5 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529960.2 | 56.6% | 39.9% | 225_228delGGTG;374_651del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 435
- ORF length:
- 369
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcccatgta gagtgccgtc ctctgggagt gtttgagtgt gaactctgta 121 ccttgacagc tccgtacagc tatgtgggac agaagccccc caacacccag tcgatggtcc 181 tcctggagga aagctatgtc atgaaggatc ccttcacctc cgacaaggac agattcctgg 241 tcctcggctc gtgctgcagt ttgtgcagca ggctggtgtg tgtgggcccg gaatgcagtt 301 tattctactc caagagattc tgcctccctt gtgtccggga gaacatcaat gcttttcctc 361 aggaaattcg gcaagacttg gagaaaagga aagctccatc aaagaggacc cccagccagc 421 ccggttctcg gacgtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 481 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 541 cgacaatcaa cctctggatt a