Construct: ORF ccsbBroad304_05060

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016597.1_s304c1, BRDN0000412013
Derived from:
ccsbBroadEn_05060
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPP4R2 (151987)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05060
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 151987 PPP4R2 protein phosphatase 4 regul... NM_174907.4 100% 100%
2 human 151987 PPP4R2 protein phosphatase 4 regul... NM_001318026.1 90.8% 90.8% 0_1ins114
3 human 151987 PPP4R2 protein phosphatase 4 regul... NM_001318025.2 86.3% 86% 116_117ins171
4 human 151987 PPP4R2 protein phosphatase 4 regul... NM_001318027.2 58.9% 54.9% 0_1ins451;41_42ins62
5 mouse 232314 Ppp4r2 protein phosphatase 4, regu... NM_182939.4 85.3% 81.9% (many diffs)
6 mouse 232314 Ppp4r2 protein phosphatase 4, regu... XM_006506008.3 75.5% 72.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1320
ORF length:
1251
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggacgtcgag aggctccagg aggcgctgaa agattttgag aagaggggga
121aaaaggaagt ttgtcctgtc ctggatcagt ttctttgtca tgtagccaag actggagaaa
181caatgattca gtggtcccaa tttaaaggct attttatttt caaactggag aaagtgatgg
241atgatttcag aacttcagct cctgagccaa gaggtcctcc caaccctaat gtcgaatata
301ttccctttga tgaaatgaag gaaagaatac tgaaaattgt cactggattt aatggtatcc
361cttttactat tcagcgacta tgtgaattgt taacagatcc aaggagaaac tatacaggaa
421cagacaaatt tctcagagga gtagaaaaga atgtgatggt tgttagctgt gtttatcctt
481cttcagagaa aaacaattcc aatagtttaa atcgaatgaa tggtgttatg tttcctggaa
541attcaccaag ctatactgag aggtctaata taaatgggcc tgggacaccc aggccactta
601atcgaccaaa ggtttctttg tcagccccca tgacaacaaa tgggttgcct gagagcacag
661acagcaaaga ggcaaatttg cagcaaaatg aggagaaaaa tcacagtgac tcttcgacct
721ctgaatcaga agtttcctca gtgagccctt tgaaaaataa acatccagat gaagatgctg
781tggaagctga ggggcatgag gtaaaaagac tcaggtttga caaagaaggt gaagtcagag
841aaacagccag tcaaacgact tccagcgaaa tttcttcagt tatggtagga gaaacagaag
901catcatcttc atctcaggat aaagacaaag atagccgttg tacccggcag cactgtacag
961aagaggatga agaagaggat gaagaggaag aagaagagtc ttttatgaca tcaagagaaa
1021tgatcccaga aagaaaaaat caagaaaaag aatctgatga tgccttaact gtgaatgaag
1081agacttctga ggaaaataat caaatggagg aatctgatgt gtctcaagct gagaaagatt
1141tgctacattc tgaaggtagt gaaaacgaag gccctgtaag tagtagttct tctgactgcc
1201gtgaaacaga agaattagta ggatccaatt ccagtaaaac tggagagatt ctttcagaat
1261catccatgga aaatgatgac gaagccacag aagtcaccga tgaaccaatg gaacaagact
1321tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1381attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1441ggatta

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