Construct: ORF ccsbBroad304_05063
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017187.1_s304c1, BRDN0000410857
- Derived from:
- ccsbBroadEn_05063
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMC1 (152100)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05063
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_182523.2 | 100% | 100% | |
| 2 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_001331187.2 | 95.7% | 92.6% | (many diffs) |
| 3 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_001331189.2 | 79.2% | 79.2% | 0_1ins66 |
| 4 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_001331186.2 | 76.8% | 76% | 110_205del |
| 5 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453370.1 | 76% | 66.6% | (many diffs) |
| 6 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_017005760.1 | 73.9% | 70.9% | (many diffs) |
| 7 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_001331190.2 | 71.6% | 71.6% | 17_18ins90 |
| 8 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NM_001331185.2 | 70.6% | 70% | 20_151del |
| 9 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453369.1 | 70.2% | 53.5% | (many diffs) |
| 10 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453371.1 | 60.8% | 60.1% | 0_1ins66;44_139del |
| 11 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453372.1 | 60.8% | 60.1% | 0_1ins66;44_139del |
| 12 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453366.1 | 58.5% | 57.4% | 20_151del;242_334del |
| 13 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453365.1 | 58.2% | 57.1% | 20_151del;242_337del |
| 14 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453368.1 | 54.4% | 43% | (many diffs) |
| 15 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XM_024453367.1 | 54.3% | 43% | (many diffs) |
| 16 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XR_002959498.1 | 37.5% | (many diffs) | |
| 17 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XR_002959495.1 | 37.4% | (many diffs) | |
| 18 | human | 152100 | CMC1 | C-X9-C motif containing 1 | XR_002959496.1 | 35.6% | (many diffs) | |
| 19 | human | 152100 | CMC1 | C-X9-C motif containing 1 | NR_138585.2 | 3.7% | (many diffs) | |
| 20 | mouse | 67899 | Cmc1 | COX assembly mitochondrial ... | NM_026442.3 | 89.3% | 88.6% | (many diffs) |
| 21 | mouse | 67899 | Cmc1 | COX assembly mitochondrial ... | XM_011242996.2 | 85.5% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gctcgacccc gcagaccagc atctcagaca tgtcgaaaaa gatgttttga 121 tccctaaaat aatgagagaa aaggccaaag agaggtgttc tgaacaagtt caagatttta 181 ccaaatgttg caagaactct ggagttctta tggtagtaaa atgccggaaa gaaaattctg 241 cattgaaaga atgtctaact gcttactata atgatccagc cttttatgaa gaatgcaaaa 301 tggaatacct gaaggaaagg gaagaattca gaaaaactgg aattcctaca aagaaaaggc 361 tacagaagct tccaacaagc atgtacccaa ctttcttgta caaagtggtt ggtaagccta 421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 481 gcgttaagtc gacaatcaac ctctggatta