Construct: ORF ccsbBroad304_05072

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000435.1_s304c1, BRDN0000403155
Derived from:
ccsbBroadEn_05072
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRFBP1 (153443)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05072
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 153443 SRFBP1 serum response factor bindi... NM_152546.3 100% 100%
2 human 153443 SRFBP1 serum response factor bindi... XM_017009111.2 86.2% 85.7% 1106G>A;1108_1108delAinsTTTCCAC;1113_1113delAins169
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1353
ORF length:
1287
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tcagccggga actctgaacc tcaataacga ggttgtgaag atgagaaaag
121aagtgaagag aattcgagtt ttagttatcc gaaaacttgt caggagtgtt ggccgactga
181agtcaaaaaa gggtactgaa gatgcactgt taaaaaacca aagacgggcg caaagattgc
241ttgaagaaat ccatgccatg aaggaattga aacctgacat agtaactaaa tctgctcttg
301gtgatgatat caactttgaa aaaatcttca aaaagccaga ttctactgca actgaaagag
361caattgccag actagcagta catcctcttc tgaagaaaaa gatagatgtg ctaaaagctg
421ctgtacaagc ctttaaagaa gcaagacaaa atgttgctga agttgagtca tcaaagaatg
481cttcagagga caatcattct gagaatactt tgtattcaaa tgataatgga agtaatttac
541agcgtgaagc aactgtcatc agtgagcaaa aagtcaaaga aaccaaaata ttggcgaaga
601aaccaataca taattcaaag gaaaaaatag caaagatgga acatggacct aaagcagtga
661ctattgcaaa ttctccatca aagccttcag aaaaggattc tgtagtttcc cttgagtccc
721agaagacacc tgctgaccca aaactgaaaa ctctaagtca aaccaaaaaa aacaaaggat
781ctgatagctc actctctggt aacagtgatg gcggagaaga attttgtgaa gaggagaagg
841aatattttga tgatagcaca gaagaaaggt tttacaagca gtcttccatg tctgaagata
901gtgatagcgg tgacgacttc ttcattggga aagtcagacg gacacgaaag aaggaaagta
961gttgtcattc ttcagttaag gaacaaaaac cactagaaaa agtgtttctt aaagaagata
1021caggtgaaac tcatggggat acaagaaatg acaaaatcaa gccaagtaca gaaaccagaa
1081agttagaatc agtgtttttc cactctttat ctggatctaa aagctctaga agaaatttca
1141aagaacaggc tccaaaaaca agatccctag attttccaca gaatgagcct cagatcaaga
1201atcagtttaa taagaagcta tcaggaagac ttgaaaatac aaaacagcaa ttgcagctgc
1261ctcttcatcc ttcatgggaa gcaagcagaa ggcgaaaaga acagcaatct aatattgctg
1321tgtttcaggg gaaaaaaatt acgtttgatg attgcccaac tttcttgtac aaagtggttg
1381gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1441accggtggcg cgttaagtcg acaatcaacc tctggatta

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