Construct: ORF ccsbBroad304_05113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003778.1_s304c1, BRDN0000411820
- Derived from:
- ccsbBroadEn_05113
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGBD4 (161779)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05113
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161779 | PGBD4 | piggyBac transposable eleme... | NM_152595.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1824
- ORF length:
- 1755
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcaaatcct agaaaacgta gcattcctat gcgtgatagt aataccggtc 121 tcgaacagtt gttggctgaa gattcatttg atgaatctga tttttcggaa atagatgatt 181 ctgataattt ttcggatagt gctttagaag ccgataagat caggcctctg tcccatttag 241 aatctgatgg aaagagctct acatcaagtg actcagggcg ctccatgaaa tggtcagctc 301 gtgctatgat tccacgtcaa aggtatgact ttaccggcac acctggcaga aaagtcgatg 361 tcagtgatat cactgaccca ttgcagtatt ttgaactgtt ctttactgag gaattagttt 421 caaaaattac tagagaaaca aatgcccaag ctgccttgtt ggcttcaaag ccaccgggtc 481 cgaaaggatt ttcgcgaatg gataaatgga aagacactga caatgacgag ctcaaagtct 541 tttttgcagt aatgttactg caaggtattg tgcagaaacc tgagctggag atgttttggt 601 caacaaggcc tcttttggat acaccttatc tcaggcaaat tatgactggt gaaagatttt 661 tacttttgtt tcggtgcctg cattttgtca acaattcttc tatatctgct ggtcaatcaa 721 aggcccagat ttcattgcag aagatcaaac ctgtgttcga ctttcttgta aataaatttt 781 ccactgtata tactccaaac agaaacattg cagttgatga atcactgatg ctgttcaagg 841 ggccattagc tatgaagcag tacctcccga caaaacgagt acgatttggt ctgaagctat 901 atgtactttg tgaaagtcag tctggttatg tgtggaatgc gcttgttcac acagggcctg 961 gcatgaattt gaaagattca gcggatggcc tgaaatcatc acgcattgtt cttaccttgg 1021 tcaatgacct tcttggccaa gggtattgtg tcttcctcga taactttaat atatctccca 1081 tgcttttcag agaattacat caaaatagga ctgatgcagt tgggacagct cgtttgaaca 1141 gaaaacagat tccaaatgat ctgaaaaaaa ggattgcaaa ggggacgact gtagccagat 1201 tctgtggtga acttatggca ctgaaatggt gtgacggcaa ggaggtgaca atgttgtcaa 1261 cattccacaa tgatactgtg attgaagtaa acaatagaaa tggaaagaaa actaaaaggc 1321 cacgtgtcat tgtggattat aacgagaata tgggagcagt ggactcggct gatcaaatgc 1381 ttacttctta tccatctgag cgcaaaagac acaaggtttg gtataagaaa ttctttcacc 1441 atcttctaca cattacagtg ctgaactcct acatcctgtt caagaaggat aatcctgagc 1501 acacgatgag ccatataaac ttcagactgg cattgattga aagaatgctg gaaaagcatc 1561 acaagccagg gcagcaacat cttcgaggtc gtccttgctc cgatgatgtc acacctcttc 1621 gtctgtctgg aagacatttc cccaagagca taccagcaac gtccgggaaa cagaatccaa 1681 ctggtcgctg caaaatttgc tgctcccaat acgacaagga tggcaagaag atccggaaag 1741 aaacgcgcta tttttgtgcc gaatgtgatg ttccgctttg tgttgttccg tgctttgaaa 1801 tttaccacac gaaaaaaaat tatttgccaa ctttcttgta caaagtggtt ggtaagccta 1861 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1921 gcgttaagtc gacaatcaac ctctggatta